In this study, we demonstrated that bcl-2 overexpression in human melanoma cells consistently enhanced the activity of multiple metastasis-related proteinases, in vitro cell invasion, and in vivo tumor growth. In particular, by using the M14 parental cell line, the MN8 control clone, and two bcl-2 overexpressing derivatives, we found that bcl-2 overexpressing cells exposed to hypoxia, when compared to parental cells, expressed higher level of several metalloproteases (MMPs) such as MMP-2, MMP-7, MT1-MMP, and tissue inhibitors of metalloproteases-1 and -2. Moreover, bcl-2 overexpression in melanoma cells enhanced in vitro invasion on matrigel and, in vivo tumor growth. The more aggressive behavior of bcl-2 transfectants tumors is significantly associated to an increase in MMP-2 expression as well as in a more elevated microvessel density as compared to the parental line. Taken together, our data suggest that bcl-2 plays a pivotal role in the regulation of molecules associated with the migratory and invasive phenotype, contributing, in cooperation to hypoxia, to tumor progression. © 2005 Wiley-Liss, Inc.

Bcl-2 overexpression in melanoma cells increases tumor progression-associated properties an in vivo tumor growth / Trisciuoglio, D; Desideri, M; Ciuffreda, L; Mottolese, M; Ribatti, D; Vacca, A; DEL ROSSO, M; Marcocci, Lucia; Zupi, G; DEL BUFALO, D.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 205:3(2005), pp. 414-421. [10.1002/jcp.20413]

Bcl-2 overexpression in melanoma cells increases tumor progression-associated properties an in vivo tumor growth

MARCOCCI, Lucia;
2005

Abstract

In this study, we demonstrated that bcl-2 overexpression in human melanoma cells consistently enhanced the activity of multiple metastasis-related proteinases, in vitro cell invasion, and in vivo tumor growth. In particular, by using the M14 parental cell line, the MN8 control clone, and two bcl-2 overexpressing derivatives, we found that bcl-2 overexpressing cells exposed to hypoxia, when compared to parental cells, expressed higher level of several metalloproteases (MMPs) such as MMP-2, MMP-7, MT1-MMP, and tissue inhibitors of metalloproteases-1 and -2. Moreover, bcl-2 overexpression in melanoma cells enhanced in vitro invasion on matrigel and, in vivo tumor growth. The more aggressive behavior of bcl-2 transfectants tumors is significantly associated to an increase in MMP-2 expression as well as in a more elevated microvessel density as compared to the parental line. Taken together, our data suggest that bcl-2 plays a pivotal role in the regulation of molecules associated with the migratory and invasive phenotype, contributing, in cooperation to hypoxia, to tumor progression. © 2005 Wiley-Liss, Inc.
2005
beta actin; gelatinase A; gelatinase B;
01 Pubblicazione su rivista::01a Articolo in rivista
Bcl-2 overexpression in melanoma cells increases tumor progression-associated properties an in vivo tumor growth / Trisciuoglio, D; Desideri, M; Ciuffreda, L; Mottolese, M; Ribatti, D; Vacca, A; DEL ROSSO, M; Marcocci, Lucia; Zupi, G; DEL BUFALO, D.. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - STAMPA. - 205:3(2005), pp. 414-421. [10.1002/jcp.20413]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/48356
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