Saliva of hematophagous insects carries a large number of factors with anti-hemostatic, antiinflammatory and immuno-modulatory activities. The Anopheles gambiae cE5 was originally identified during a salivary gland transcriptome study and later predicted to encode a salivary inhibitor of thrombin. Its expression pattern, not restricted to female glands, and the existence of cE5 transcripts carrying 3’UTRs of different length suggested that cE5 may have some additional function. We expressed/purified the An. gambiae cE5 and we show here that it is a specific, tight and fast-binding inhibitor of thrombin. Expression analysis by RT-PCR and 3’RACE revealed that cE5 transcripts of different length are found in several tissues of both male and female mosquitoes; however, western blot analysis failed to detect the cE5 protein in any tissue analyzed but female salivary glands. In conclusion, our study shows that the An. gambiae cE5 is a thrombin inhibitor, that the cE5 gene is post-transcriptionally regulated and that transcriptomic data should be interpreted with caution in the absence of proteomic support.

Unusual expression profile of cE5, an Anopheles gambiae salivary polypeptide with anti-thrombin activity / R., Ronca; M., Kotsyfakis; Lombardo, Fabrizio; Rizzo, Cinzia; C., Curra'; M., Ponzi; G., Fiorentino; J. M. C., Ribeiro; Arca', Bruno. - STAMPA. - (2012), pp. 7-7. (Intervento presentato al convegno IV Annual Meeting COST Action BM0802 Life or Death of Protozoan Parasites – V Annual meeting Italian Malaria Network (IMN) CIRM-Centro Universitario Ricerca sulla Malaria tenutosi a Milano (Italy) nel 19-21 Gennaio 2012).

Unusual expression profile of cE5, an Anopheles gambiae salivary polypeptide with anti-thrombin activity

LOMBARDO, Fabrizio;RIZZO, CINZIA;ARCA', Bruno
2012

Abstract

Saliva of hematophagous insects carries a large number of factors with anti-hemostatic, antiinflammatory and immuno-modulatory activities. The Anopheles gambiae cE5 was originally identified during a salivary gland transcriptome study and later predicted to encode a salivary inhibitor of thrombin. Its expression pattern, not restricted to female glands, and the existence of cE5 transcripts carrying 3’UTRs of different length suggested that cE5 may have some additional function. We expressed/purified the An. gambiae cE5 and we show here that it is a specific, tight and fast-binding inhibitor of thrombin. Expression analysis by RT-PCR and 3’RACE revealed that cE5 transcripts of different length are found in several tissues of both male and female mosquitoes; however, western blot analysis failed to detect the cE5 protein in any tissue analyzed but female salivary glands. In conclusion, our study shows that the An. gambiae cE5 is a thrombin inhibitor, that the cE5 gene is post-transcriptionally regulated and that transcriptomic data should be interpreted with caution in the absence of proteomic support.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/481945
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