Prostaglandin D synthetase (PGD-S; prostaglandin-H2 D-isomerase, EC 5,3,99,2), a 30 kDa glycoprotein also known as beta-trace protein that catalyzes the formation of prostaglandin D2 (PGD2) from PGH2, was purified to apparent homogeneity from human cerebrospinal fluid (CSF) using a two-step procedure involving HPLC on a Vydac C8 reversed-phase column and high performance electrophoresis chromatography (HPEC) using a 10% T SDS-polyacrylamide gel. The purity of PGD-S isolated from CSF was confirmed by silver stained SDS-polyacrylamide gel and direct protein microsequencing (NH2-APEAQVSVQPNFQ). A highly specific polyclonal antibody was prepared against this protein for immunoassay development. Using an ELISA, it was found that the concentration of PGD-S in CSF did not alter significantly in different pathological conditions of the central nervous system (CNS). These include dementia (n = 9), hydrocephalus (n = 4), neuropathy (n = 11), optic neuritis (n = 4), multiple sclerosis (n = 11), and demyelinating syndrome (n = 11), when compared to normal individuals (n = 12); however, the level of PGD-S in the CSF obtained from patients with brain tumor (n = 11), was reduced by as much as 2-fold when compared to control samples (n = 12) illustrating PGD-S is a potentially useful marker for brain tumor.
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|Titolo:||1998 Nov;46(4): Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor.|
|Data di pubblicazione:||1998|
|Citazione:||1998 Nov;46(4): Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor. / Saso, Luciano; Leone, Mg; Sorrentino, C; Giacomelli, S; Silvestrini, B; Grima, J; Li, Jc; Samy, E; Mruk, D; Cheng, Cy. - In: BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL. - ISSN 1039-9712. - 46(1998), pp. 643-56.|
|Appare nella tipologia:||01a Articolo in rivista|