Background: The pathogenesis of spondyloarthropathies (SpAs), a group of chronic inflammatory rheumatic disorders, is still unknown, but recently a prominent role for Natural Killer (NK) cells has been advanced. The aim of this study was to analyze the function of peripheral blood NK cells from SpA patients focusing on their cytotoxic properties and cytokine production, particularly TNF and IFN. Methods: We enrolled fifteen out-patients with SpAs , 7 with ankylosing spondylitis, 6 with psoriatic arthritis, 2 with undifferentiated SpA and 6 healthy donors. Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood by Lymphoprep gradient centrifugation. Using multi-parameter flow cytometry, NK cell cytotoxicity was assessed through a degranulation assay which measures cell-surface expression of the lysosomal protein CD107a (LAMP-1) in PBMCs incubated with either K562, an erythroleukemia cell line, or the mouse mastocytoma cell line P815 pre-incubated with antibodies directed against NK-cell activating receptors CD16 and NKG2D (CD314). The intra-cellular production of TNF and IFN following 6 hrs PBMCs stimulation with P815 cells pre-incubated with anti-CD16, anti-NKG2D or both antibodies, was quantified using multi-parameter flow cytometry. Results: NK cell natural and antibody-stimulated cytotoxicity, with P815 pre-incubated with both anti-CD16 and anti-NKG2D antibodies, were increased compared to healthy donors. A positive correlation was found between cytotoxic abilities and TNF and IFN production following stimulation of NK cells with P815 pre-incubated with anti-CD16 (p<0.03) and with both antibodies (p<0.02). Conclusions: The increased cytotoxic activity, together with the cytokine production, indicate an activated status of NK cells in the patients studied , supporting a pathogenetic role for this lymphocyte population in SpA.

Functional characterization of peripheral blood natural killer cells in spondyloarthropathy patients / Scrivo, Rossana; Morrone, Stefania; Spadaro, Antonio; M., Vespaziani; Valesini, Guido; Santoni, Angela. - In: MINERVA MEDICA. - ISSN 0026-4806. - STAMPA. - 98 (Suppl. 1):(2007), pp. 50-50. (Intervento presentato al convegno 5th National Conference SIICA tenutosi a Trieste, ITALY nel June 6-9, 2007).

Functional characterization of peripheral blood natural killer cells in spondyloarthropathy patients.

SCRIVO, Rossana;MORRONE, Stefania;SPADARO, Antonio;VALESINI, Guido;SANTONI, Angela
2007

Abstract

Background: The pathogenesis of spondyloarthropathies (SpAs), a group of chronic inflammatory rheumatic disorders, is still unknown, but recently a prominent role for Natural Killer (NK) cells has been advanced. The aim of this study was to analyze the function of peripheral blood NK cells from SpA patients focusing on their cytotoxic properties and cytokine production, particularly TNF and IFN. Methods: We enrolled fifteen out-patients with SpAs , 7 with ankylosing spondylitis, 6 with psoriatic arthritis, 2 with undifferentiated SpA and 6 healthy donors. Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood by Lymphoprep gradient centrifugation. Using multi-parameter flow cytometry, NK cell cytotoxicity was assessed through a degranulation assay which measures cell-surface expression of the lysosomal protein CD107a (LAMP-1) in PBMCs incubated with either K562, an erythroleukemia cell line, or the mouse mastocytoma cell line P815 pre-incubated with antibodies directed against NK-cell activating receptors CD16 and NKG2D (CD314). The intra-cellular production of TNF and IFN following 6 hrs PBMCs stimulation with P815 cells pre-incubated with anti-CD16, anti-NKG2D or both antibodies, was quantified using multi-parameter flow cytometry. Results: NK cell natural and antibody-stimulated cytotoxicity, with P815 pre-incubated with both anti-CD16 and anti-NKG2D antibodies, were increased compared to healthy donors. A positive correlation was found between cytotoxic abilities and TNF and IFN production following stimulation of NK cells with P815 pre-incubated with anti-CD16 (p<0.03) and with both antibodies (p<0.02). Conclusions: The increased cytotoxic activity, together with the cytokine production, indicate an activated status of NK cells in the patients studied , supporting a pathogenetic role for this lymphocyte population in SpA.
2007
5th National Conference SIICA
spondyloarthropathies (SpAs); rheumatic disorders; medicine
04 Pubblicazione in atti di convegno::04c Atto di convegno in rivista
Functional characterization of peripheral blood natural killer cells in spondyloarthropathy patients / Scrivo, Rossana; Morrone, Stefania; Spadaro, Antonio; M., Vespaziani; Valesini, Guido; Santoni, Angela. - In: MINERVA MEDICA. - ISSN 0026-4806. - STAMPA. - 98 (Suppl. 1):(2007), pp. 50-50. (Intervento presentato al convegno 5th National Conference SIICA tenutosi a Trieste, ITALY nel June 6-9, 2007).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/481241
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