Background: Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Here, we have asked whether variations in the constitutive expression of early-growth response factor 1 (EGR-1) predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics.Materials and methods: Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant glioma. In addition to EGR-1, tumor cultures were investigated for genetic alterations and the expression of cancer regulating factors, related to the p53 pathway.Results: We found that sensitivity to cisplatin correlates significantly with levels of EGR-1 expression in tumors with wild-type p53/INK4a/p16 status.Conclusion: Increased knowledge of the mechanisms regulating EGR-1 expression in wild-type p53/INK4a/p16 cases of glioma may help in the design of new chemotherapeutic strategies for these tumors. © 2011 Calogero et al; licensee BioMed Central Ltd.
Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression / Calogero, Antonella; Antonio, Porcellini; Vincenza, Lombari; Fabbiano, Cinzia; Arcella, Antonietta; Miscusi, Massimo; Ponti, Donatella; Ragona, Giuseppe. - In: CANCER CELL INTERNATIONAL. - ISSN 1475-2867. - ELETTRONICO. - 11:(2011), p. 5. [10.1186/1475-2867-11-5]
Sensitivity to cisplatin in primary cell lines derived from human glioma correlates with levels of EGR-1 expression
CALOGERO, ANTONELLA;FABBIANO, CINZIA;ARCELLA, ANTONIETTA;MISCUSI, MASSIMO;PONTI, Donatella;RAGONA, Giuseppe
2011
Abstract
Background: Less than 30% of malignant gliomas respond to adjuvant chemotherapy. Here, we have asked whether variations in the constitutive expression of early-growth response factor 1 (EGR-1) predicted acute cytotoxicity and clonogenic cell death in vitro, induced by six different chemotherapics.Materials and methods: Cytotoxicity assays were performed on cells derived from fresh tumor explants of 18 human cases of malignant glioma. In addition to EGR-1, tumor cultures were investigated for genetic alterations and the expression of cancer regulating factors, related to the p53 pathway.Results: We found that sensitivity to cisplatin correlates significantly with levels of EGR-1 expression in tumors with wild-type p53/INK4a/p16 status.Conclusion: Increased knowledge of the mechanisms regulating EGR-1 expression in wild-type p53/INK4a/p16 cases of glioma may help in the design of new chemotherapeutic strategies for these tumors. © 2011 Calogero et al; licensee BioMed Central Ltd.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
