p _0.029). CTCs were found in 48/108 patients(44%); the unfavourable group was that with evidence of recurrent disease(RD) or progressive disease (PD). Tumoral Survivin expression and presence ofCTC were correlated to DFS. Multivariate analysis was used to investigate whetherCTC presence was independent indicator of DFS.Results: Survivin was found in 50% of tumors. Survivin - patients showed a longerDFS than Survivin _ (_2: 4.572; 92% of CTC were Survivin expressing. The difference in DFS betweenCTC - and CTC _ patients was statistically significant (_2: 28.098; p _0.001). CTCpresence was found an independent prognostic factor of DFS (p_0.001).Conclusions: CTC presence is an independent prognostic factor in high riskNMIBC patients. The importance of the study will be confirmed by the use ofVeridex equipment for the exact count of the CTS.; absenceof CIS and multifocality. Planned follow up was 24 mo. Survivin was evaluatedby RT-PCR in tumoral tissues. CTCs were isolated from blood by CELLection™Dynabeads coated with the monoclonal antibody towards the human EpithelialCell Adhesion Molecule. Cells were lysed and Dynabeads Oligo(dT) was used tocapture poly A_ mRNA. cDNA was synthesised and analysed for the expression ofCD45, CK8 and Survivin. The primary end point was disease free survival (DFS);the favourable group at 24 mo was defined as that without any clinical evidence ofdisease (NED); Introduction & Objectives: The prognosis of T1G3 bladder cancer is highlyvariable and not predictable basing upon clinical and pathological prognosticfactors. There is need for improvement in risk stratification in this population;understanding the molecular profile of individual patients could provide a morepersonalized and tailored treatment. Main objective was to evaluate the prognosticsignificance of Survivin in tumor tissues and that of Survivin expressingcirculatingtumor cells (CTCs) in T1G3 tumors.Materials & Methods: 108 patients with T1G3 non muscle invasive bladder cancer(NMIBC) were enrolled. Additional inclusion criteria were: tumor size_3cm
EVALUATION AND PROGNOSTIC FACTOR OF CIRCULATING TUMOR CELL IN NMIBC (NON-MUSCLE INVASIVE BLADDER CANCER) / DE BERARDINIS, Ettore; Busetto, GIAN MARIA; Sciarra, Alessandro; Cristini, Cristiano; A., Petracca; Nicolazzo, Chiara; Gazzaniga, Paola; Gentile, Vincenzo. - In: EUROPEAN UROLOGY. SUPPLEMENTS. - ISSN 1569-9056. - STAMPA. - 10:2(2011), pp. 196-196. (Intervento presentato al convegno 26th Annual Congress of the European Association of Urology tenutosi a Vienna nel 2010) [10.1016/s1569-9056(11)60590-3].
EVALUATION AND PROGNOSTIC FACTOR OF CIRCULATING TUMOR CELL IN NMIBC (NON-MUSCLE INVASIVE BLADDER CANCER)
DE BERARDINIS, Ettore;BUSETTO, GIAN MARIA;SCIARRA, Alessandro;CRISTINI, Cristiano;NICOLAZZO , CHIARA;GAZZANIGA, PAOLA;GENTILE, Vincenzo
2011
Abstract
p _0.029). CTCs were found in 48/108 patients(44%); the unfavourable group was that with evidence of recurrent disease(RD) or progressive disease (PD). Tumoral Survivin expression and presence ofCTC were correlated to DFS. Multivariate analysis was used to investigate whetherCTC presence was independent indicator of DFS.Results: Survivin was found in 50% of tumors. Survivin - patients showed a longerDFS than Survivin _ (_2: 4.572; 92% of CTC were Survivin expressing. The difference in DFS betweenCTC - and CTC _ patients was statistically significant (_2: 28.098; p _0.001). CTCpresence was found an independent prognostic factor of DFS (p_0.001).Conclusions: CTC presence is an independent prognostic factor in high riskNMIBC patients. The importance of the study will be confirmed by the use ofVeridex equipment for the exact count of the CTS.; absenceof CIS and multifocality. Planned follow up was 24 mo. Survivin was evaluatedby RT-PCR in tumoral tissues. CTCs were isolated from blood by CELLection™Dynabeads coated with the monoclonal antibody towards the human EpithelialCell Adhesion Molecule. Cells were lysed and Dynabeads Oligo(dT) was used tocapture poly A_ mRNA. cDNA was synthesised and analysed for the expression ofCD45, CK8 and Survivin. The primary end point was disease free survival (DFS);the favourable group at 24 mo was defined as that without any clinical evidence ofdisease (NED); Introduction & Objectives: The prognosis of T1G3 bladder cancer is highlyvariable and not predictable basing upon clinical and pathological prognosticfactors. There is need for improvement in risk stratification in this population;understanding the molecular profile of individual patients could provide a morepersonalized and tailored treatment. Main objective was to evaluate the prognosticsignificance of Survivin in tumor tissues and that of Survivin expressingcirculatingtumor cells (CTCs) in T1G3 tumors.Materials & Methods: 108 patients with T1G3 non muscle invasive bladder cancer(NMIBC) were enrolled. Additional inclusion criteria were: tumor size_3cmI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
