The circular plasmid pKD1 (or 1.6 um DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 u DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology, pKD1 can be transferred toKluyverornyces lactis where it is replicated stably. Using recombinant molecules derived from pKD1, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2 u-based Saccharomyces cerevisiae transformation system.
Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 um circular plasmid pKD1 / Bianchi, Michele Maria; Falcone, Claudio; Chen, X. J.; WESOLOWSKI LOUVEL, M.; Frontali, Laura; Fukuhara, H.. - In: CURRENT GENETICS. - ISSN 0172-8083. - 12:(1987), pp. 185-192.
Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 um circular plasmid pKD1
BIANCHI, Michele Maria;FALCONE, Claudio;FRONTALI, Laura;
1987
Abstract
The circular plasmid pKD1 (or 1.6 um DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 u DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology, pKD1 can be transferred toKluyverornyces lactis where it is replicated stably. Using recombinant molecules derived from pKD1, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2 u-based Saccharomyces cerevisiae transformation system.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
