The circular plasmid pKD1 (or 1.6 um DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 u DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology, pKD1 can be transferred toKluyverornyces lactis where it is replicated stably. Using recombinant molecules derived from pKD1, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2 u-based Saccharomyces cerevisiae transformation system.

Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 um circular plasmid pKD1 / Bianchi, Michele Maria; Falcone, Claudio; Chen, X. J.; WESOLOWSKI LOUVEL, M.; Frontali, Laura; Fukuhara, H.. - In: CURRENT GENETICS. - ISSN 0172-8083. - 12(1987), pp. 185-192.

Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 um circular plasmid pKD1

BIANCHI, Michele Maria;FALCONE, Claudio;FRONTALI, Laura;
1987

Abstract

The circular plasmid pKD1 (or 1.6 um DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 u DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology, pKD1 can be transferred toKluyverornyces lactis where it is replicated stably. Using recombinant molecules derived from pKD1, a practical transformation system has been developed for Kluyveromyces lactis, with an efficiency and stability comparable to the 2 u-based Saccharomyces cerevisiae transformation system.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/47634
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