A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 u/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1-based DNA bank of K. lactis, The KEX1 gene involved in the killer system was isolated by complementation
A gene cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production / Chen, X. J.; WESOLOWSKI LOUVEL, M.; TANGUY ROUGEAU, C.; Bianchi, Michele Maria; Fabiani, Lucia; Saliola, Michele; Falcone, Claudio; Frontali, Laura; Fukuhara, H.. - In: JOURNAL OF BASIC MICROBIOLOGY. - ISSN 0233-111X. - 28:(1988), pp. 211-220.
A gene cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production
BIANCHI, Michele Maria;FABIANI, Lucia;SALIOLA, Michele;FALCONE, Claudio;FRONTALI, Laura;
1988
Abstract
A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 u/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1-based DNA bank of K. lactis, The KEX1 gene involved in the killer system was isolated by complementationI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
