A selective method for measuring tricyclic antidepressants in serum is reported. A single assay can be done within ca. 30 min and eight samples can be assayed in less than 150 min. A 1-ml serum sample was diluted and the drugs were extracted from it by passage through a graphitized carbon black (Carbopack B) cartridge. After one washing, this cartridge was connected on line to another one containing a silica-based strong acid exchange. The tricyclics were removed from the Carbopack surface and selectively readsorbed onto the cation-exchange surface by passing 4 ml of methylene chloride-methanol (60:40, v/v) through the two cartridges. After another wash, the drugs were desorbed from the cation-exchange surface with 0.8 ml of acetonitrile-methanol-water (72:18:10, v/v) saturated with potassium chloride. An aliquot of this solution was chromatographed on a cyano column, and the absorbance of the effluent was measured at 215 nm. The mean analytical recoveries of tricyclic antidepressants added to serum within the range 10-200 μg/l exceeded 90%, except for 8-hydroxyamoxapine (mean recovery 85.3%) and amoxapine (mean recovery 83.8%) at the lowest serum concentration considered.

Antidepressants in serum determined by isolation with two on-line sorbent cartridges and liquid chromatography / Guia, Carfagnini; DI CORCIA, Antonio; Marcello, Marchetti; Samperi, Roberto. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - STAMPA. - 530:2(1990), pp. 359-366. [10.1016/s0378-4347(00)82338-5]

Antidepressants in serum determined by isolation with two on-line sorbent cartridges and liquid chromatography

DI CORCIA, Antonio;SAMPERI, Roberto
1990

Abstract

A selective method for measuring tricyclic antidepressants in serum is reported. A single assay can be done within ca. 30 min and eight samples can be assayed in less than 150 min. A 1-ml serum sample was diluted and the drugs were extracted from it by passage through a graphitized carbon black (Carbopack B) cartridge. After one washing, this cartridge was connected on line to another one containing a silica-based strong acid exchange. The tricyclics were removed from the Carbopack surface and selectively readsorbed onto the cation-exchange surface by passing 4 ml of methylene chloride-methanol (60:40, v/v) through the two cartridges. After another wash, the drugs were desorbed from the cation-exchange surface with 0.8 ml of acetonitrile-methanol-water (72:18:10, v/v) saturated with potassium chloride. An aliquot of this solution was chromatographed on a cyano column, and the absorbance of the effluent was measured at 215 nm. The mean analytical recoveries of tricyclic antidepressants added to serum within the range 10-200 μg/l exceeded 90%, except for 8-hydroxyamoxapine (mean recovery 85.3%) and amoxapine (mean recovery 83.8%) at the lowest serum concentration considered.
1990
01 Pubblicazione su rivista::01a Articolo in rivista
Antidepressants in serum determined by isolation with two on-line sorbent cartridges and liquid chromatography / Guia, Carfagnini; DI CORCIA, Antonio; Marcello, Marchetti; Samperi, Roberto. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - STAMPA. - 530:2(1990), pp. 359-366. [10.1016/s0378-4347(00)82338-5]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/473367
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