A 0.35 mM concn. of lonidamine produced a 60% inhibition of the replication of T. lewisi in culture. Administered to Fischer rats infected with T. lewisi, lonidamine provoked a 30% decrease in the no. of parasites in the blood on the 5th day of infection. Electron microscopy studies (showing damage to the mitochondrial membrane) and the analyses of phosphorylated metabolites by 31P-NMR spectroscopy (decrease of nucleotide triphosphate and nucleotide dephosphate levels) suggested the presumed interference of lonidamine in the energy metab. of trypanosomes. The presence of 2-amino-3-phosphonopropionic acid and 2-aminoethylphosphonic acid phosphonates in T. lewisi trypomastigotes were also revealed by means of 31P-NMR.
Trypanosoma lewisi: study of the activity of lonidamine in vitro and in vivo in rats / M., Manganaro; M. R., Luparini; R., Ricciolini; F., Caliò; Miccheli, Alfredo; F., Conti. - In: CELLULAR AND MOLECULAR BIOLOGY. - ISSN 0145-5680. - STAMPA. - 34:5(1988), pp. 517-528.
Trypanosoma lewisi: study of the activity of lonidamine in vitro and in vivo in rats
MICCHELI, Alfredo;
1988
Abstract
A 0.35 mM concn. of lonidamine produced a 60% inhibition of the replication of T. lewisi in culture. Administered to Fischer rats infected with T. lewisi, lonidamine provoked a 30% decrease in the no. of parasites in the blood on the 5th day of infection. Electron microscopy studies (showing damage to the mitochondrial membrane) and the analyses of phosphorylated metabolites by 31P-NMR spectroscopy (decrease of nucleotide triphosphate and nucleotide dephosphate levels) suggested the presumed interference of lonidamine in the energy metab. of trypanosomes. The presence of 2-amino-3-phosphonopropionic acid and 2-aminoethylphosphonic acid phosphonates in T. lewisi trypomastigotes were also revealed by means of 31P-NMR.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
