In primary cultures of myoblasts from embryonic chick skeletal muscle, cells undergo a period of proliferation lasting 2 days, and then fuse into multinucleated myotubes. It is a generally accepted idea that cell fusion represents a critical event followed by the synthesis of specific proteins. It was reported that no specific enzymes are synthesized by mononucleated myoblasts (COLEMANan d CoLEhiAN, 1968; SHAINBEReGt ul., 1971; LOOMIS el al., 1973). We investigated the relationship between cell fusion and the activity of specific enzymes, in order to verify whether the expression of these phenomena is linked to a common regulative signal. The activity of creatine kinase (CPK; E.C. 2. 7. 3 2.) has been investigated in culture conditions which prevent cell fusion without affecting cell growth. These culture conditions consisted of the use of a nutritional medium at low serum concentration (MOLINAROet al., 1974). The inhibition of cell fusion obtained by low serum concentration depends primarily on the reduced Ca++ concentration, since the addition of Ca++ restores cell fusion.
Cell fusion and creatine kinase activity in cultures of chick embryo myoblasts / Adamo, Sergio; Curci, Armando; Molinaro, Mario; Gregorio, Siracusa; Valerio, Monesi. - In: BOLLETTINO DI ZOOLOGIA. - ISSN 0373-4137. - 42:1(1975), pp. 49-56. [10.1080/11250007509430130]
Cell fusion and creatine kinase activity in cultures of chick embryo myoblasts
ADAMO, Sergio;CURCI, Armando;MOLINARO, Mario;
1975
Abstract
In primary cultures of myoblasts from embryonic chick skeletal muscle, cells undergo a period of proliferation lasting 2 days, and then fuse into multinucleated myotubes. It is a generally accepted idea that cell fusion represents a critical event followed by the synthesis of specific proteins. It was reported that no specific enzymes are synthesized by mononucleated myoblasts (COLEMANan d CoLEhiAN, 1968; SHAINBEReGt ul., 1971; LOOMIS el al., 1973). We investigated the relationship between cell fusion and the activity of specific enzymes, in order to verify whether the expression of these phenomena is linked to a common regulative signal. The activity of creatine kinase (CPK; E.C. 2. 7. 3 2.) has been investigated in culture conditions which prevent cell fusion without affecting cell growth. These culture conditions consisted of the use of a nutritional medium at low serum concentration (MOLINAROet al., 1974). The inhibition of cell fusion obtained by low serum concentration depends primarily on the reduced Ca++ concentration, since the addition of Ca++ restores cell fusion.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.