Abstract: High levels of mRNA (as assessed by northern blot) for the high-affinity nerve growth factor receptor (p140(TRK)) are predictive of favourable outcome in neuroblastoma. The feasibility of determining p140(trk) on frozen sections using a recently developed monoclonal antibody was evaluated, and immunohistochemical findings were compared to those obtained from northern blot analysis. Primary tumour samples from 28 untreated patients were quick frozen and an indirect immunofluorescence assay was performed on 4-mu m acetone-fixed cryostat sections. 9 cases were positive with immunohistochemistry, and these were among the 15 cases also positive by northern blot. None of the cases negative by northern blot were positive with immunohistochemistry. The concordance rate was 79% (P < 0.03), with a sensitivity of 60% and a specificity of 100%. Immunohistochemistry can thus be rather reliable for assessing p140(trk) expression, even when only very small amounts of tissue are available, such as with needle biopsy.
Immunohistochemical detection of high-affinity nerve growth factor receptor in neuroblastoma / Dominici, Carlo; M. R., Nicotra; G., Digiesi; H., Mcdowell; S., Alemà; A., Padula; N., Gargano; A., Donfrancesco; M. A., Castello; P. G., Natali. - In: EUROPEAN JOURNAL OF CANCER. - ISSN 0959-8049. - STAMPA. - 31:(1995), pp. 444-446. [10.1016/0959-8049(95)00065-Q]
Immunohistochemical detection of high-affinity nerve growth factor receptor in neuroblastoma
DOMINICI, Carlo;
1995
Abstract
Abstract: High levels of mRNA (as assessed by northern blot) for the high-affinity nerve growth factor receptor (p140(TRK)) are predictive of favourable outcome in neuroblastoma. The feasibility of determining p140(trk) on frozen sections using a recently developed monoclonal antibody was evaluated, and immunohistochemical findings were compared to those obtained from northern blot analysis. Primary tumour samples from 28 untreated patients were quick frozen and an indirect immunofluorescence assay was performed on 4-mu m acetone-fixed cryostat sections. 9 cases were positive with immunohistochemistry, and these were among the 15 cases also positive by northern blot. None of the cases negative by northern blot were positive with immunohistochemistry. The concordance rate was 79% (P < 0.03), with a sensitivity of 60% and a specificity of 100%. Immunohistochemistry can thus be rather reliable for assessing p140(trk) expression, even when only very small amounts of tissue are available, such as with needle biopsy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
