Kinetic properties of the oxidation of several Met-enkephalins and of kyotorphin by tyrosinase are reported. The opioid peptides tested present an affinity for the enzyme equal or higher than tyrosine itself. Long lasting incubations of these opioid peptides with tyrosinase lead to the formation of DOPA, the presence of ascorbic acid improving the recovery of the aminoacid. Spectrophotometric measurements in the absence of ascorbic acid indicate the formation of dopachrome which reaches the maximum within 15-30 minutes.
TYROSINASE-CATALYZED OXIDATION OF MET-ENKEPHALINS / M. A., Rosei; Antonilli, Letizia; Coccia, Raffaella; Blarzino, Carla. - In: PHYSIOLOGICAL CHEMISTRY AND PHYSICS AND MEDICAL NMR. - ISSN 0748-6642. - STAMPA. - 23:(1991), pp. 59-65.
TYROSINASE-CATALYZED OXIDATION OF MET-ENKEPHALINS
ANTONILLI, Letizia;COCCIA, Raffaella;BLARZINO, Carla
1991
Abstract
Kinetic properties of the oxidation of several Met-enkephalins and of kyotorphin by tyrosinase are reported. The opioid peptides tested present an affinity for the enzyme equal or higher than tyrosine itself. Long lasting incubations of these opioid peptides with tyrosinase lead to the formation of DOPA, the presence of ascorbic acid improving the recovery of the aminoacid. Spectrophotometric measurements in the absence of ascorbic acid indicate the formation of dopachrome which reaches the maximum within 15-30 minutes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
