A kinetic model was developed which describes the unsteady behaviour of liposomes entrapping the enzyme urease. The model allows to determine how the reactant concentrations, the internal pH and the catalytically active enzyme fraction vary with time. A numerical simulation carried out to elucidate the effects of the buffer pH and ionic strength on the active enzyme decay provided useful directions for the selection of the operating conditions to be used for the removal of urea by urease-loaded liposomes.
Urease-loaded liposomes as detoxifying microreactors: Effect of ammonia accumulation on enzyme kinetics / F., Cioci; Lavecchia, Roberto. - In: JOURNAL OF LIPOSOME RESEARCH. - ISSN 0898-2104. - 3:3(1993), pp. 725-735.
Urease-loaded liposomes as detoxifying microreactors: Effect of ammonia accumulation on enzyme kinetics
LAVECCHIA, Roberto
1993
Abstract
A kinetic model was developed which describes the unsteady behaviour of liposomes entrapping the enzyme urease. The model allows to determine how the reactant concentrations, the internal pH and the catalytically active enzyme fraction vary with time. A numerical simulation carried out to elucidate the effects of the buffer pH and ionic strength on the active enzyme decay provided useful directions for the selection of the operating conditions to be used for the removal of urea by urease-loaded liposomes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
