We have compared in the same cell type the surface distribution and partition in freeze-fractured plasma membranes of Sindbis virus glycoproteins in three different situations: (i) in permanently transformed cells that express the glycoproteins as the only viral product; (ii) in cells in which pre-bound viruses were forced to fuse with the plasma membrane by low pH treatment; (iii) in virus-infected cells. We report here that the viral proteins expressed on the surface of transfected cells show a uniform and unclustered distribution; conversely, in Sindbis virus-infected cells they appear clustered, regionally distributed, and always associated with budding viruses (i.e., interacting with the nucleocapsid on the cytosolic side of the membrane). Furthermore, the viral proteins expressed on transfected cells or implanted by low pH-mediated fusion partition during freeze-fracture with the exoplasmic faces of the cell plasma membranes, whereas an opposite partition is observed in infected cells. These results strongly suggest that in infected cells the clustering and the partition with the protoplasmic faces of the plasma membrane depend only on the strong 'anchorage' of the glycoproteins to the nucleocapsid.

ANCHORAGE-DEPENDENT SURFACE DISTRIBUTION AND PARTITION DURING FREEZE-FRACTURE OF VIRAL TRANSMEMBRANE GLYCOPROTEINS / Torrisi, Maria Rosaria; A., Pavan; Lotti, Lavinia Vittoria; G., Migliaccio; M. C., Pascale; E., Covelli; A., Leone; S., Bonatti. - In: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. - ISSN 0022-1554. - STAMPA. - 38:10(1990), pp. 1421-1426. [10.1177/38.10.2401782]

ANCHORAGE-DEPENDENT SURFACE DISTRIBUTION AND PARTITION DURING FREEZE-FRACTURE OF VIRAL TRANSMEMBRANE GLYCOPROTEINS

TORRISI, Maria Rosaria;LOTTI, Lavinia Vittoria;
1990

Abstract

We have compared in the same cell type the surface distribution and partition in freeze-fractured plasma membranes of Sindbis virus glycoproteins in three different situations: (i) in permanently transformed cells that express the glycoproteins as the only viral product; (ii) in cells in which pre-bound viruses were forced to fuse with the plasma membrane by low pH treatment; (iii) in virus-infected cells. We report here that the viral proteins expressed on the surface of transfected cells show a uniform and unclustered distribution; conversely, in Sindbis virus-infected cells they appear clustered, regionally distributed, and always associated with budding viruses (i.e., interacting with the nucleocapsid on the cytosolic side of the membrane). Furthermore, the viral proteins expressed on transfected cells or implanted by low pH-mediated fusion partition during freeze-fracture with the exoplasmic faces of the cell plasma membranes, whereas an opposite partition is observed in infected cells. These results strongly suggest that in infected cells the clustering and the partition with the protoplasmic faces of the plasma membrane depend only on the strong 'anchorage' of the glycoproteins to the nucleocapsid.
freeze-fracture immunocytochemistry; membranes; sindbis virus; viral proteins
01 Pubblicazione su rivista::01a Articolo in rivista
ANCHORAGE-DEPENDENT SURFACE DISTRIBUTION AND PARTITION DURING FREEZE-FRACTURE OF VIRAL TRANSMEMBRANE GLYCOPROTEINS / Torrisi, Maria Rosaria; A., Pavan; Lotti, Lavinia Vittoria; G., Migliaccio; M. C., Pascale; E., Covelli; A., Leone; S., Bonatti. - In: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. - ISSN 0022-1554. - STAMPA. - 38:10(1990), pp. 1421-1426. [10.1177/38.10.2401782]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/470805
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