A linear plasmid was constructed in vitro using the telomeres of the rDNA of Tetrahymena pyriformis. These telomeres were added to a yeast circular vector containing an ARS sequence from Dictyostelium, the LEU2 gene of yeast and the neo gene from Escherichia coli Tn5 fused with a eukaryotic promoter. The resulting plasmid was used to transform yeast. During the replication of the linear plasmid in yeast it was spontaneously modified at the extremity by the addition of 300 bp of yeast telomeric sequence for each end. Total DNA prepared from yeast transformants was used to transform the hypotrichous ciliate Stylonychia lemnae. The same plasmid isolated from Stylonychia can again be replicated in yeast.

A LINEAR SHUTTLE VECTOR FOR YEAST AND THE HYPOTRICHOUS CILIATE STYLONYCHIA / Ascenzioni, Fiorentina; Hans J., Lipps. - In: GENE. - ISSN 0378-1119. - 46:1(1986), pp. 123-126. [10.1016/0378-1119(86)90174-5]

A LINEAR SHUTTLE VECTOR FOR YEAST AND THE HYPOTRICHOUS CILIATE STYLONYCHIA

ASCENZIONI, Fiorentina;
1986

Abstract

A linear plasmid was constructed in vitro using the telomeres of the rDNA of Tetrahymena pyriformis. These telomeres were added to a yeast circular vector containing an ARS sequence from Dictyostelium, the LEU2 gene of yeast and the neo gene from Escherichia coli Tn5 fused with a eukaryotic promoter. The resulting plasmid was used to transform yeast. During the replication of the linear plasmid in yeast it was spontaneously modified at the extremity by the addition of 300 bp of yeast telomeric sequence for each end. Total DNA prepared from yeast transformants was used to transform the hypotrichous ciliate Stylonychia lemnae. The same plasmid isolated from Stylonychia can again be replicated in yeast.
1986
01 Pubblicazione su rivista::01a Articolo in rivista
A LINEAR SHUTTLE VECTOR FOR YEAST AND THE HYPOTRICHOUS CILIATE STYLONYCHIA / Ascenzioni, Fiorentina; Hans J., Lipps. - In: GENE. - ISSN 0378-1119. - 46:1(1986), pp. 123-126. [10.1016/0378-1119(86)90174-5]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/470726
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact