A variant clone of Chinese hamster ovary (CHO) cells resistant to thialysine has been isolated. It maintains the phenotypic properties even after 250 generations in medium without thialysine. Growth rate, cell viability and protein synthesis rate of the variant are much less affected by thialysine than the parental strain. In both the parental strain and the variant, thialysine acts in competition with lysine as indicated by the fact that all thialysine effects can be completely reversed by lysine. © 1986.
ISOLATION AND PROPERTIES OF A THIALYSINE-RESISTANT CLONE OF CHO CELLS / M. D., Girolamo; A. D., Girolamo; C., Foppoli; Blarzino, Carla; C. D., Marco. - In: MUTATION RESEARCH. MUTATION RESEARCH LETTERS. - ISSN 0165-7992. - STAMPA. - 173:3(1986), pp. 223-227. [10.1016/0165-7992(86)90040-0]
ISOLATION AND PROPERTIES OF A THIALYSINE-RESISTANT CLONE OF CHO CELLS
BLARZINO, Carla;
1986
Abstract
A variant clone of Chinese hamster ovary (CHO) cells resistant to thialysine has been isolated. It maintains the phenotypic properties even after 250 generations in medium without thialysine. Growth rate, cell viability and protein synthesis rate of the variant are much less affected by thialysine than the parental strain. In both the parental strain and the variant, thialysine acts in competition with lysine as indicated by the fact that all thialysine effects can be completely reversed by lysine. © 1986.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
