Cytochrome c oxidase was reconstituted in phospholipid vesicles in the presence of highly hydrophobic poly(vinyl alkanoate) polymers. Electron-microscopy observations demonstrated that polymer interaction with the lipid phase induces vesicles to adopt smaller diameters than those typical of standard proteoliposomes. Functional characterization of these polymer-proteoliposome structures indicates that the reconstitution of the enzyme proceeds efficiently without causing either scrambling of the protein orientation in the membrane or loss of respiratory control. A clear dependence of respiratory control ratio on vesicle size was also demonstrated, which is in agreement with a previous model proposed for control of activity of cytochrome c oxidase vesicles
Reconstitution of cytochrome c oxidase in phospholipid vesicles containing polyvinylic polymers / Sarti, Paolo; Antonini, G; Malatesta, Francesco; Vallone, Beatrice; Villaschi, S; Brunori, Maurizio; Hider, Rc; Hamed, K.. - STAMPA. - 257:(1989), pp. 783-787.
Reconstitution of cytochrome c oxidase in phospholipid vesicles containing polyvinylic polymers.
SARTI, Paolo;MALATESTA, FRANCESCO;VALLONE, Beatrice;BRUNORI, Maurizio;
1989
Abstract
Cytochrome c oxidase was reconstituted in phospholipid vesicles in the presence of highly hydrophobic poly(vinyl alkanoate) polymers. Electron-microscopy observations demonstrated that polymer interaction with the lipid phase induces vesicles to adopt smaller diameters than those typical of standard proteoliposomes. Functional characterization of these polymer-proteoliposome structures indicates that the reconstitution of the enzyme proceeds efficiently without causing either scrambling of the protein orientation in the membrane or loss of respiratory control. A clear dependence of respiratory control ratio on vesicle size was also demonstrated, which is in agreement with a previous model proposed for control of activity of cytochrome c oxidase vesiclesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.