Lanthionine ketimine (LK), cystathionine ketimine (CK) and thialisine ketimine (AECK) represent a new class of cyclic sulfur-containing iminoacids recently identified in the CNS and humane urine. These natural compounds have been synthesized and chemically characterized in our laboratories. In order to define the role of these iminoacids as putative neuromodulators we made ready the synthesis and the purification of [35S]LK. Binding experiments performed on bovine cerebral membranes indicate the presence of a ligand site for LK, exhibiting the basic criteria of saturability and reversibility typical of a receptor-ligand system. Scatchard analysis indicates the presence of a single population of sites with an affinity constant of about 30 nM; binding is maximal between pH 7-7.5 and it is scarcely affected by ionic strength and ionic composition. Funher studies are in progress to characterize the specificity of binding by displacement experiments in the precence of known neuroactive agonists or antagonists.

Binding of [35S]lanthionine ketimine to bovine brain membranes / Ricci, G.; Vesci, L.; Corsico, N.; Fontana, Mario; Dupre', Silvestro; Arrigoni Martelli, E.; Cavallini, Doriano. - In: PHARMACOLOGICAL RESEARCH. - ISSN 1043-6618. - STAMPA. - 22(1990), pp. 428-428. ((Intervento presentato al convegno The XXV National Congress of the Italian Society of Pharmacology tenutosi a Taormina (ME) nel 14-18 ottobre 1990 [10.1016/S1043-6618(09)80438-6].

Binding of [35S]lanthionine ketimine to bovine brain membranes.

G. Ricci;FONTANA, Mario;DUPRE', Silvestro;CAVALLINI, Doriano
1990

Abstract

Lanthionine ketimine (LK), cystathionine ketimine (CK) and thialisine ketimine (AECK) represent a new class of cyclic sulfur-containing iminoacids recently identified in the CNS and humane urine. These natural compounds have been synthesized and chemically characterized in our laboratories. In order to define the role of these iminoacids as putative neuromodulators we made ready the synthesis and the purification of [35S]LK. Binding experiments performed on bovine cerebral membranes indicate the presence of a ligand site for LK, exhibiting the basic criteria of saturability and reversibility typical of a receptor-ligand system. Scatchard analysis indicates the presence of a single population of sites with an affinity constant of about 30 nM; binding is maximal between pH 7-7.5 and it is scarcely affected by ionic strength and ionic composition. Funher studies are in progress to characterize the specificity of binding by displacement experiments in the precence of known neuroactive agonists or antagonists.
File allegati a questo prodotto
File Dimensione Formato  
Ricci_Binding_1990.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 65.55 kB
Formato Adobe PDF
65.55 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/467916
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact