2H-1,4-Thiazine-5,6-dihydro-3,5-dicarboxylic acid (trivial name: lanthionine ketimine) is a cyclic sulfur-containing imino acid detected in bovine brain extracts. This compound has been synthesized in a heavily labeled form starting from L-[35S]cysteine and purified by high performance liquid chromatography. We demonstrate the existence of a saturable and reversible binding of [35S]lanthionine ketimine to bovine brain membranes. A single population of binding sites with a concentration of 260 ± 12 fmol/mg protein and a dissociation constant of 58 ± 14 nM is present. Specific binding is competitively inhibited by other structurally similar imino acids, namely S-aminoethyl-L-cysteine ketimine and cystathionine ketimine. These results suggest a possible functional role for these ketimines in nervous system.

[35S]Lanthionine ketimine binding to bovine brain membranes / Fontana, Mario; Ricci, G; Solinas, Sp; Antonucci, A; Serao, I; Dupre', Silvestro; Cavallini, Doriano. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 171(1990), pp. 480-486. [10.1016/0006-291X(90)91418-R]

[35S]Lanthionine ketimine binding to bovine brain membranes.

FONTANA, Mario;DUPRE', Silvestro;CAVALLINI, Doriano
1990

Abstract

2H-1,4-Thiazine-5,6-dihydro-3,5-dicarboxylic acid (trivial name: lanthionine ketimine) is a cyclic sulfur-containing imino acid detected in bovine brain extracts. This compound has been synthesized in a heavily labeled form starting from L-[35S]cysteine and purified by high performance liquid chromatography. We demonstrate the existence of a saturable and reversible binding of [35S]lanthionine ketimine to bovine brain membranes. A single population of binding sites with a concentration of 260 ± 12 fmol/mg protein and a dissociation constant of 58 ± 14 nM is present. Specific binding is competitively inhibited by other structurally similar imino acids, namely S-aminoethyl-L-cysteine ketimine and cystathionine ketimine. These results suggest a possible functional role for these ketimines in nervous system.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/467890
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