Spectroscopic measurements in single living cells are made possible by the development of computer controlled light detectors, which, when applied to optical microscopes, yield spatial, temporal and eventually spectroscopic information about the sample. This minireview describes some experiments in which the distribution and concentration of specific intracellular markers (proteins, protein complexes, RNA) has been followed by quantitative microscopy. The examples chosen have contributed to shed light on a biochemical process as it happens in vivo; because of the non ideal conditions of the intracellular milieu, the comparison of in vivo and in vitro experiments is of great relevance to the understanding of cellular physiology.

ANALYSIS OF BIOCHEMICAL PROCESSES IN SINGLE LIVING CELLS BY QUANTITATIVE MICROSCOPY / Bellelli, Andrea; R., Ippoliti; Lendaro, Eugenio; Brunori, Maurizio. - In: BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL. - ISSN 1039-9712. - STAMPA. - 33:2(1994), pp. 289-311.

ANALYSIS OF BIOCHEMICAL PROCESSES IN SINGLE LIVING CELLS BY QUANTITATIVE MICROSCOPY

BELLELLI, Andrea;LENDARO, Eugenio;BRUNORI, Maurizio
1994

Abstract

Spectroscopic measurements in single living cells are made possible by the development of computer controlled light detectors, which, when applied to optical microscopes, yield spatial, temporal and eventually spectroscopic information about the sample. This minireview describes some experiments in which the distribution and concentration of specific intracellular markers (proteins, protein complexes, RNA) has been followed by quantitative microscopy. The examples chosen have contributed to shed light on a biochemical process as it happens in vivo; because of the non ideal conditions of the intracellular milieu, the comparison of in vivo and in vitro experiments is of great relevance to the understanding of cellular physiology.
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ANALYSIS OF BIOCHEMICAL PROCESSES IN SINGLE LIVING CELLS BY QUANTITATIVE MICROSCOPY / Bellelli, Andrea; R., Ippoliti; Lendaro, Eugenio; Brunori, Maurizio. - In: BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL. - ISSN 1039-9712. - STAMPA. - 33:2(1994), pp. 289-311.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/466575
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