Thialysine cannot completely substitute lysine as growth factor for a lysine-requiring E. coli mutant. However it can be utilized for growth in the presence of limiting amounts of lysine, in substitution of, and in competition with this latter. The effects of thialysine on growth rate, protein synthesis rate and cell viability, and its incorporation into proteins were studied in function of lysine and thialysine concentration in the culture media. Up to 60% of protein lysine substitution by thialysine is observed, without appreciable effects on cell viability.
Thialysine utilization by a lysine-requiring Escherichia coli mutant / Cini, Chiara; V., Busiello; Coccia, Raffaella; M. D., Girolamo; C. D., Marco. - In: MOLECULAR AND CELLULAR BIOCHEMISTRY. - ISSN 0300-8177. - STAMPA. - 59:1-2(1984), pp. 149-154.
Thialysine utilization by a lysine-requiring Escherichia coli mutant.
CINI, Chiara;COCCIA, Raffaella;
1984
Abstract
Thialysine cannot completely substitute lysine as growth factor for a lysine-requiring E. coli mutant. However it can be utilized for growth in the presence of limiting amounts of lysine, in substitution of, and in competition with this latter. The effects of thialysine on growth rate, protein synthesis rate and cell viability, and its incorporation into proteins were studied in function of lysine and thialysine concentration in the culture media. Up to 60% of protein lysine substitution by thialysine is observed, without appreciable effects on cell viability.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
