A selective RIA for D-Ala2-Dermorphin (Der), a natural peptide extracted from amphibian skin, has been developed using an antibody raised in rabbits against Der which has been coupled to BSA through its phenolic hydroxyl groups of tyrosine residues with 2,4-Dichloro-6-methoxy-1,3,5-triazine. The cross-reactivity of this antibody with dermorphin analogs, C- and N-terminal fragments of dermorphin molecule, some opioid and gastrointestinal peptides was tested. Der-like immunoreactivity has been identified in tissue extracts of rats, frog and cephalopoda. Der-like peptides were purified by passing methanol extracts of the tissues through a Sephadex G25 column (16x100 cm) eluted with 0.1 M acetic acid at 4°C. Der-like immunoreactivity from neural tissue of Dosidicus gigas, Eledone moscata, and rat brain showed a good agreement with an authentic sample of synthetic dermorphin.

Radioimmunoassay of dermorphin-like peptides in mammalian and non-mammalian tissues / Negri, Lucia; Melchiorri, Pietro; G. F., Erspamer; V., Erspamer. - In: PEPTIDES. - ISSN 0196-9781. - STAMPA. - 2:Suppl. 2(1981), pp. 45-49.

Radioimmunoassay of dermorphin-like peptides in mammalian and non-mammalian tissues

NEGRI, Lucia;MELCHIORRI, Pietro;
1981

Abstract

A selective RIA for D-Ala2-Dermorphin (Der), a natural peptide extracted from amphibian skin, has been developed using an antibody raised in rabbits against Der which has been coupled to BSA through its phenolic hydroxyl groups of tyrosine residues with 2,4-Dichloro-6-methoxy-1,3,5-triazine. The cross-reactivity of this antibody with dermorphin analogs, C- and N-terminal fragments of dermorphin molecule, some opioid and gastrointestinal peptides was tested. Der-like immunoreactivity has been identified in tissue extracts of rats, frog and cephalopoda. Der-like peptides were purified by passing methanol extracts of the tissues through a Sephadex G25 column (16x100 cm) eluted with 0.1 M acetic acid at 4°C. Der-like immunoreactivity from neural tissue of Dosidicus gigas, Eledone moscata, and rat brain showed a good agreement with an authentic sample of synthetic dermorphin.
1981
01 Pubblicazione su rivista::01a Articolo in rivista
Radioimmunoassay of dermorphin-like peptides in mammalian and non-mammalian tissues / Negri, Lucia; Melchiorri, Pietro; G. F., Erspamer; V., Erspamer. - In: PEPTIDES. - ISSN 0196-9781. - STAMPA. - 2:Suppl. 2(1981), pp. 45-49.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/466137
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