Release of both D-[3H]aspartate and endogenous amino acids was measured in primary cultures of cerebellar granule cells. Two hour-pretreatment with the glycosphingolipids, GM1 or GT1b, attenuated the stimulation of excitatory amino acid release induced by depolarizing concentrations of K+ (50 mM). Gangliosides inhibited the phencyclidine (PCP)-sensitive component of depolarization-induced release, i.e. the amplification of release that follows activation of NMDA receptors by the endogenous glutamate.
Gangliosides attenuate NMDA receptor-mediated excitatory amino acid release in cultured cerebellar neurons / Nicoletti, F., S., C., Bruno, V.M.G., M., V., M. V., C., A., C., P. L., C.. - In: NEUROPHARMACOLOGY. - ISSN 0028-3908. - 28:(1989), pp. 1283-1286. [10.1016/0028-3908(89)90224-4]
Gangliosides attenuate NMDA receptor-mediated excitatory amino acid release in cultured cerebellar neurons.
NICOLETTI, Ferdinando;BRUNO, Valeria Maria Gloria;
1989
Abstract
Release of both D-[3H]aspartate and endogenous amino acids was measured in primary cultures of cerebellar granule cells. Two hour-pretreatment with the glycosphingolipids, GM1 or GT1b, attenuated the stimulation of excitatory amino acid release induced by depolarizing concentrations of K+ (50 mM). Gangliosides inhibited the phencyclidine (PCP)-sensitive component of depolarization-induced release, i.e. the amplification of release that follows activation of NMDA receptors by the endogenous glutamate.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
