The iron chelator desferrioxamine reversibly inhibits heme accumulation and globin synthesis in human leukemic K-562 cells induced to express erythroid genes by butyric acid. These results suggest that iron metabolism can modulate globin gene expression. In addition we describe experimental conditions (6.25 μg/ml desferrioxamine) which do not suppress transcription of globin genes and translation of globin mRNA but prevent heme synthesis. Therefore expression of globin genes in butyric acid induced K-562 cells does not require accumulation of heme molecules. Human leukemic K-562 cells cultured with different inducers and treated with desferrioxamine should be used as a useful model system to further analyse the relationship between expression of erythroid genes, iron metabolism and heme accumulation. © 1983.

Desferrioxamine inhibits induced erythroid differentiation of human leukemic K-562 cells / R., Gambari; G., Raschella; Tripodi, Marco; M. G., Farace; A., Fantoni. - In: CELL DIFFERENTIATION. - ISSN 0045-6039. - STAMPA. - 12:5(1983), pp. 249-255.

Desferrioxamine inhibits induced erythroid differentiation of human leukemic K-562 cells

TRIPODI, Marco;
1983

Abstract

The iron chelator desferrioxamine reversibly inhibits heme accumulation and globin synthesis in human leukemic K-562 cells induced to express erythroid genes by butyric acid. These results suggest that iron metabolism can modulate globin gene expression. In addition we describe experimental conditions (6.25 μg/ml desferrioxamine) which do not suppress transcription of globin genes and translation of globin mRNA but prevent heme synthesis. Therefore expression of globin genes in butyric acid induced K-562 cells does not require accumulation of heme molecules. Human leukemic K-562 cells cultured with different inducers and treated with desferrioxamine should be used as a useful model system to further analyse the relationship between expression of erythroid genes, iron metabolism and heme accumulation. © 1983.
1983
desferrioxamine; erythroid differentiation; k-562 cells
01 Pubblicazione su rivista::01a Articolo in rivista
Desferrioxamine inhibits induced erythroid differentiation of human leukemic K-562 cells / R., Gambari; G., Raschella; Tripodi, Marco; M. G., Farace; A., Fantoni. - In: CELL DIFFERENTIATION. - ISSN 0045-6039. - STAMPA. - 12:5(1983), pp. 249-255.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/465778
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