In this article we show that the cytidine analog 5-azacytidine is able to induce differentiation of the human leukemia K-562 cell line. Erythroid induction is associated with (a) an increase of the overall globin synthesis and globin mRNA accumulation, (b) a relative increase of fetal with respect to embryonic globins, and (c) a decrease of the proliferative capacity of hemoglobin-containing cells. In addition, we have analysed the DNA methylation pattern at the cleavage sites of MspI and HpaII restriction enzymes, which are known to cleave differently CCGG DNA sequences when 5-methylcytosine is present. These experiments indicate that in K-562 cells treated with 5-azacytidine, DNA becomes hypomethylated, suggesting that genetic programmes leading to an erythroid phenotype may be activated by a reduction of DNA methylation. © 1984.

Human leukemia K-562 cells: induction of erythroid differentiation by 5-azacytidine / R., Gambari; L., Del Senno; R., Barbieri; L., Viola; Tripodi, Marco; G., Raschella; A., Fantoni. - In: CELL DIFFERENTIATION. - ISSN 0045-6039. - STAMPA. - 14:2(1984), pp. 87-97.

Human leukemia K-562 cells: induction of erythroid differentiation by 5-azacytidine

TRIPODI, Marco;
1984

Abstract

In this article we show that the cytidine analog 5-azacytidine is able to induce differentiation of the human leukemia K-562 cell line. Erythroid induction is associated with (a) an increase of the overall globin synthesis and globin mRNA accumulation, (b) a relative increase of fetal with respect to embryonic globins, and (c) a decrease of the proliferative capacity of hemoglobin-containing cells. In addition, we have analysed the DNA methylation pattern at the cleavage sites of MspI and HpaII restriction enzymes, which are known to cleave differently CCGG DNA sequences when 5-methylcytosine is present. These experiments indicate that in K-562 cells treated with 5-azacytidine, DNA becomes hypomethylated, suggesting that genetic programmes leading to an erythroid phenotype may be activated by a reduction of DNA methylation. © 1984.
1984
5-azacytidine; dna methylation; erythroid differentiation; globin mrna; k-562 cell
01 Pubblicazione su rivista::01a Articolo in rivista
Human leukemia K-562 cells: induction of erythroid differentiation by 5-azacytidine / R., Gambari; L., Del Senno; R., Barbieri; L., Viola; Tripodi, Marco; G., Raschella; A., Fantoni. - In: CELL DIFFERENTIATION. - ISSN 0045-6039. - STAMPA. - 14:2(1984), pp. 87-97.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/465773
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