α-1-antitrypsin (α-1AT) present in large amounts in human serum and. synthesized predominantly in hepatocytes, is the most abundant protease inhibitor and α-1AT mutant proteins are associated with heriditary disorders. To investigate the regulation of the normal human α-1AT gene, we have microinjected fertilized mouse eggs with a 17.5 kb DNA fragment containing the entire gene with 7 kb 5′ and 0.3 kb 3′ flanking sequences. We show that this DNA fragment contains all the information for efficient, accurate and tissue-specific expression. High serum concentration of the human protein was found in three independent transgenic mouse lines. The human α-1AT RNA is transcribed efficiently in liver, kidney and macrophages and we demonstrate that two different promoters are used for the expression in liver and macrophages of the transgenic mice. © 1987 IRL Press Limited, Oxford, England.
The human alpha-1-antitrypsin gene is efficiently expressed from two tissue-specific promotors in transgenic mice / Rüther, Ulrich; Tripodi, Marco; Riccardo, Cortcsc; F., Wagner Erwin. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 15:18(1987), pp. 7519-7529. [10.1093/nar/15.18.7519]
The human alpha-1-antitrypsin gene is efficiently expressed from two tissue-specific promotors in transgenic mice
TRIPODI, Marco;
1987
Abstract
α-1-antitrypsin (α-1AT) present in large amounts in human serum and. synthesized predominantly in hepatocytes, is the most abundant protease inhibitor and α-1AT mutant proteins are associated with heriditary disorders. To investigate the regulation of the normal human α-1AT gene, we have microinjected fertilized mouse eggs with a 17.5 kb DNA fragment containing the entire gene with 7 kb 5′ and 0.3 kb 3′ flanking sequences. We show that this DNA fragment contains all the information for efficient, accurate and tissue-specific expression. High serum concentration of the human protein was found in three independent transgenic mouse lines. The human α-1AT RNA is transcribed efficiently in liver, kidney and macrophages and we demonstrate that two different promoters are used for the expression in liver and macrophages of the transgenic mice. © 1987 IRL Press Limited, Oxford, England.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
