The mouse Htf9-a gene encodes a small hydrophilic protein, named Ran-binding protein 1 (RanBP-1), for its interaction with the Ras-related Ran protein; RanBP-1 binds the biologically active form of Ran. Ran has been implicated in a variety of nuclear events including DNA replication, RNA export and protein import, and monitoring completion of DNA synthesis before the onset of mitosis; its biological activity is thought to be regulated in S phase. We have investigated whether expression of the Htf9-a/RanBP-1 gene is linked to cell proliferation. Expression of the Htf9-a/RanBP-1 transcript appeared to be dependent on the proliferating state of the cells and peaked in S phase. We have identified a promoter region required for Htf9-a cell cycle-dependent expression and for responsiveness to cell cycle regulators. An E2F-binding site was identified within the cell cycle-regulated promoter region. Expression of the E1A oncoprotein prevented Htf9-a down-regulation in quiescent cells; in addition, both pRb and its relative p107 inhibited transcription from the Htf9-a promoter. These results link the control of Htf9a/RanBP-1 expression to the cell cycle progression.

Transcriptional control of the Htf9-A/RanBP-1 gene during the cell cycle / Di Matteo, G; Fuschi, P; Zerfass, K; Moretti, S; Ricordy, R; Cenciarelli, C; Tripodi, Marco; Jansen Durr, P; Lavia, P.. - In: CELL GROWTH & DIFFERENTIATION. - ISSN 1044-9523. - STAMPA. - 6:(1995), pp. 1213-1224.

Transcriptional control of the Htf9-A/RanBP-1 gene during the cell cycle.

TRIPODI, Marco;
1995

Abstract

The mouse Htf9-a gene encodes a small hydrophilic protein, named Ran-binding protein 1 (RanBP-1), for its interaction with the Ras-related Ran protein; RanBP-1 binds the biologically active form of Ran. Ran has been implicated in a variety of nuclear events including DNA replication, RNA export and protein import, and monitoring completion of DNA synthesis before the onset of mitosis; its biological activity is thought to be regulated in S phase. We have investigated whether expression of the Htf9-a/RanBP-1 gene is linked to cell proliferation. Expression of the Htf9-a/RanBP-1 transcript appeared to be dependent on the proliferating state of the cells and peaked in S phase. We have identified a promoter region required for Htf9-a cell cycle-dependent expression and for responsiveness to cell cycle regulators. An E2F-binding site was identified within the cell cycle-regulated promoter region. Expression of the E1A oncoprotein prevented Htf9-a down-regulation in quiescent cells; in addition, both pRb and its relative p107 inhibited transcription from the Htf9-a promoter. These results link the control of Htf9a/RanBP-1 expression to the cell cycle progression.
1995
01 Pubblicazione su rivista::01a Articolo in rivista
Transcriptional control of the Htf9-A/RanBP-1 gene during the cell cycle / Di Matteo, G; Fuschi, P; Zerfass, K; Moretti, S; Ricordy, R; Cenciarelli, C; Tripodi, Marco; Jansen Durr, P; Lavia, P.. - In: CELL GROWTH & DIFFERENTIATION. - ISSN 1044-9523. - STAMPA. - 6:(1995), pp. 1213-1224.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/465671
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