We present the structure of four precursors for [D-Ala2ljdeltorphins I and Has deduced from cDNAs cloned from skin of the frog PhyUomedusa bwolor. These contain the genetic information for one copy of [D-Ala2Jdeltorphin H and zero, one, or three copies of [D-Ala2jdeltorphin I. In each case, the D-alanine of the end product is encoded by a normal GCG codon for L-alanine. In addition, the existence of three peptides related to dermorphin was predicted from the amino acid sequence of the precursors. These peptides were synthesized with a D-alanine in position 2 and their pharmacological properties were tested. Two of them, [Lys7Jdermorphin-OH and [Trp4,Asn7Jdermorphin-OH, were found to have roughly the same affinity and selectivity for Ip-type opioid receptors as dermorphin.

CDNAs encoding [D-Ala2]deltorphin precursors from skin of Phyllomedusa bicolor also contain genetic information for three dermorphin-related opioid peptides / K., Richter; R., Egger; Negri, Lucia; R., Corsi; C., Severini; G., Kreil. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 87:12(1990), pp. 4836-4839. [10.1073/pnas.87.12.4836]

CDNAs encoding [D-Ala2]deltorphin precursors from skin of Phyllomedusa bicolor also contain genetic information for three dermorphin-related opioid peptides

NEGRI, Lucia;
1990

Abstract

We present the structure of four precursors for [D-Ala2ljdeltorphins I and Has deduced from cDNAs cloned from skin of the frog PhyUomedusa bwolor. These contain the genetic information for one copy of [D-Ala2Jdeltorphin H and zero, one, or three copies of [D-Ala2jdeltorphin I. In each case, the D-alanine of the end product is encoded by a normal GCG codon for L-alanine. In addition, the existence of three peptides related to dermorphin was predicted from the amino acid sequence of the precursors. These peptides were synthesized with a D-alanine in position 2 and their pharmacological properties were tested. Two of them, [Lys7Jdermorphin-OH and [Trp4,Asn7Jdermorphin-OH, were found to have roughly the same affinity and selectivity for Ip-type opioid receptors as dermorphin.
1990
amphibian skin peptides; precursors
01 Pubblicazione su rivista::01a Articolo in rivista
CDNAs encoding [D-Ala2]deltorphin precursors from skin of Phyllomedusa bicolor also contain genetic information for three dermorphin-related opioid peptides / K., Richter; R., Egger; Negri, Lucia; R., Corsi; C., Severini; G., Kreil. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 87:12(1990), pp. 4836-4839. [10.1073/pnas.87.12.4836]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/463388
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