The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photobleaching recovery, is D = 1 - 2 x 10 -10 cm 2/s. Fluorescence microscopy provides preliminary evidence for secondary endosomes in the cytoplasm.

Binding and internalization of ricin labelled with fluorescein isothiocyanate / Bellelli, Andrea; R., Ippoliti; Brunori, Maurizio; Z., Kam; M., Benveniste; F., Emmanuel; E., Turpin; A., Alfsen; J. P., Frénoy. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 169:(1990), pp. 602-609. [10.1016/0006-291X(90)90373-U]

Binding and internalization of ricin labelled with fluorescein isothiocyanate

BELLELLI, Andrea;BRUNORI, Maurizio;
1990

Abstract

The toxic lectin ricin has been covalently labelled with fluorescein isothiocyanate on the enzymatically active A chain. The fluorescein reacted toxin maintains its biological activity. The lateral diffusion coefficient of cell surface bound ricin, studied in two cell lines by fluorescence photobleaching recovery, is D = 1 - 2 x 10 -10 cm 2/s. Fluorescence microscopy provides preliminary evidence for secondary endosomes in the cytoplasm.
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Binding and internalization of ricin labelled with fluorescein isothiocyanate / Bellelli, Andrea; R., Ippoliti; Brunori, Maurizio; Z., Kam; M., Benveniste; F., Emmanuel; E., Turpin; A., Alfsen; J. P., Frénoy. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 169:(1990), pp. 602-609. [10.1016/0006-291X(90)90373-U]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/463309
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