Previous biochemical and pharmacological studies have shown that [D-Ala2]-deltorphin-I (DADT) has high affinity and selectivity for δ-opioid receptors. In this study, designed to provide morphological details, the distribution of DADTI binding sites was examined by autoradiography on coronal, sagittal and horizontal frozen sections of adult rat brain. The sections were incubated with tritiated DADTI solution and exposed for 12 weeks to a 3H-sensitive film. DADTI labelling clearly demonstrated selective and high affinity binding sites of δ-opioid type in several brain regions, including olfactory system, neostriatum, nucleus accumbens, and cortical layers I-II and V-VI.
Autoradiographic study on [3H]-[D-Ala2]-deltorphin-I binding sites in the rat brain / T., Renda; Negri, Lucia; I., Tooyama; C., Casu; Melchiorri, Pietro. - In: NEUROREPORT. - ISSN 0959-4965. - STAMPA. - 4:10(1993), pp. 1143-1146.
Autoradiographic study on [3H]-[D-Ala2]-deltorphin-I binding sites in the rat brain
NEGRI, Lucia;MELCHIORRI, Pietro
1993
Abstract
Previous biochemical and pharmacological studies have shown that [D-Ala2]-deltorphin-I (DADT) has high affinity and selectivity for δ-opioid receptors. In this study, designed to provide morphological details, the distribution of DADTI binding sites was examined by autoradiography on coronal, sagittal and horizontal frozen sections of adult rat brain. The sections were incubated with tritiated DADTI solution and exposed for 12 weeks to a 3H-sensitive film. DADTI labelling clearly demonstrated selective and high affinity binding sites of δ-opioid type in several brain regions, including olfactory system, neostriatum, nucleus accumbens, and cortical layers I-II and V-VI.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
