13C and 31P NMR parameters were obtained for phosphomycin in aq. soln. The pH dependence of chem. shifts was used to calc. the pK of the 2nd deprotonation process (pK2 = 6.4). The pH dependence of the 13C-31P and 31P-1H coupling consts. pointed to a change in the dihedral angles and hence in the geometry of phosphomycin during deprotonation. The pH dependences of 31P spin-lattice relaxation rates and 31P-{1H} NOEs were interpreted in terms of relative wt. of the 31P-1H dipolar interaction in detg. the relaxation mechanism.
Carbon-13 and phosphorus-31 NMR studies of phosphomycin, a phosphonate antibiotic / Delfini, Maurizio; DI COCCO, Maria Enrica; M. R., Del Giudice; E., Gaggelli; G., Valensin; D., Marini. - In: SPECTROSCOPY LETTERS. - ISSN 0038-7010. - STAMPA. - 22:4(1989), pp. 363-374. [10.1080/00387018908053886]
Carbon-13 and phosphorus-31 NMR studies of phosphomycin, a phosphonate antibiotic
DELFINI, Maurizio;DI COCCO, Maria Enrica;
1989
Abstract
13C and 31P NMR parameters were obtained for phosphomycin in aq. soln. The pH dependence of chem. shifts was used to calc. the pK of the 2nd deprotonation process (pK2 = 6.4). The pH dependence of the 13C-31P and 31P-1H coupling consts. pointed to a change in the dihedral angles and hence in the geometry of phosphomycin during deprotonation. The pH dependences of 31P spin-lattice relaxation rates and 31P-{1H} NOEs were interpreted in terms of relative wt. of the 31P-1H dipolar interaction in detg. the relaxation mechanism.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
