A detailed investigation with affinity-chromatography-purified fractions of antihemoglobin serum from rabbit shows that the hemoglobin content of human bones dating back 15 to 3,000 years may be very small. Some of the previous results (Ascenzi et al., 1985) indicating a high hemoglobin titer were vitiated because of an unexpected cross-reactivity of bone extracts with the hemoglobin-unreactive fraction of the antiserum. This study calls attention to possible artifacts intrinsic to the application of immunological techniques to the study of archaeological samples.
Brief communication: On the problem of immunological detection of antigens in skeletal remains / Lendaro, Eugenio; R., Ippoliti; Bellelli, Andrea; Brunori, Maurizio; R., Zito; G., Citro; Ascenzi, Antonio. - In: AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY. - ISSN 0002-9483. - STAMPA. - 86:3(1991), pp. 429-432. [10.1002/ajpa.1330860308]