Utilization of thialysine and selenalysine for protein synthesis by a lysine requiring E. coli mutant was studied. Incorporation into proteins of thialysine or selenalysine, added to culture medium together with lysine, becomes evident when the amount of available lysine in the medium is highly reduced, that is the mutant utilizes the isologs only after all the available natural aminoacid has been utilized. Compared to selenalysine, thialysine is better utilized; when both isologs are present in the medium at equal concentrations, up to 46\% of protein lysine is substituted by thialysine and only 12\% by selenalysine.
Thialysine and selenalysine utilization for protein synthesis by E. coli in comparison with lysine / Coccia, Raffaella; V., Busiello; Cini, Chiara; C., Foppoli; M. D., Girolamo. - In: ITALIAN JOURNAL OF BIOCHEMISTRY. - ISSN 0021-2938. - STAMPA. - 32:(1983), pp. 19-27.
Thialysine and selenalysine utilization for protein synthesis by E. coli in comparison with lysine.
COCCIA, Raffaella;CINI, Chiara;
1983
Abstract
Utilization of thialysine and selenalysine for protein synthesis by a lysine requiring E. coli mutant was studied. Incorporation into proteins of thialysine or selenalysine, added to culture medium together with lysine, becomes evident when the amount of available lysine in the medium is highly reduced, that is the mutant utilizes the isologs only after all the available natural aminoacid has been utilized. Compared to selenalysine, thialysine is better utilized; when both isologs are present in the medium at equal concentrations, up to 46\% of protein lysine is substituted by thialysine and only 12\% by selenalysine.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.