Chinese Hamster Ovary (CHO) cells utilize thialysine when added to the culture medium. Thialysine utilization is prevented by increasing lysine concentration in the medium, thus indicating that thialysine is utilized in substitution for and in competition with lysine. Almost all thialysine disappeared from the medium is recovered in cell protein hydrolysates. Thialysine is used for protein synthesis in substitution for lysine, and up to 10\% of lysine can be substituted.
Thialysine utilization for protein synthesis by CHO cells / M. D., Girolamo; A. D., Girolamo; Cini, Chiara; Coccia, Raffaella; C. D., Marco. - In: PHYSIOLOGICAL CHEMISTRY AND PHYSICS AND MEDICAL NMR. - ISSN 0748-6642. - STAMPA. - 18:(1986), pp. 159-164.
Thialysine utilization for protein synthesis by CHO cells.
CINI, Chiara;COCCIA, Raffaella;
1986
Abstract
Chinese Hamster Ovary (CHO) cells utilize thialysine when added to the culture medium. Thialysine utilization is prevented by increasing lysine concentration in the medium, thus indicating that thialysine is utilized in substitution for and in competition with lysine. Almost all thialysine disappeared from the medium is recovered in cell protein hydrolysates. Thialysine is used for protein synthesis in substitution for lysine, and up to 10\% of lysine can be substituted.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
