High-performance liquid chromatography (HPLC) was employed for the separation and determination of impurities [5-benzoyl-2-acetylthiophene (BAT), 5-benzoyl-2-ethylthiophene (BET) and (RS)-5-benzoyl-alpha-methyl-3-thiopheneacetic acid (3-isomer of tiaprofenic acid) contained in bulk racemic tiaprofenic acid and pharmaceutical formulations. Chiral columns containing the 3,5-dimethylphenylcarbamate of cellulose and amylose were used. The effect of the organic modifier, 2-propanol, in the mobile phase was studied. The HPLC method gave good performances from qualitative and a quantitative standpoints, allowing the enantiomeric ratios of tiaprofenic acid and its 3-isomer to be determined together with the related impurities BAT and BET.
Direct High-performance Liquid-chromatography Resolution On Chiral Columns of Tiaprofenic Acid and Related-compounds In Bulk Powder and Pharmaceutical Formulations / R., Ferretti; B., Gallinella; Villani, Claudio. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 704:(1995), pp. 217-223. [10.1016/0021-9673(94)00027-7]
Direct High-performance Liquid-chromatography Resolution On Chiral Columns of Tiaprofenic Acid and Related-compounds In Bulk Powder and Pharmaceutical Formulations
VILLANI, Claudio
1995
Abstract
High-performance liquid chromatography (HPLC) was employed for the separation and determination of impurities [5-benzoyl-2-acetylthiophene (BAT), 5-benzoyl-2-ethylthiophene (BET) and (RS)-5-benzoyl-alpha-methyl-3-thiopheneacetic acid (3-isomer of tiaprofenic acid) contained in bulk racemic tiaprofenic acid and pharmaceutical formulations. Chiral columns containing the 3,5-dimethylphenylcarbamate of cellulose and amylose were used. The effect of the organic modifier, 2-propanol, in the mobile phase was studied. The HPLC method gave good performances from qualitative and a quantitative standpoints, allowing the enantiomeric ratios of tiaprofenic acid and its 3-isomer to be determined together with the related impurities BAT and BET.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
