We generated FM7a and CyO balancer chromosomes bearing a Tubby(1) (Tb(1)) dominant transgene. Flies heterozygous for these FM7a and CyO derivatives exhibit a phenotype undistinguishable from that elicited by the Tb1 mutation associated with the TM6B balancer. We tested two of these Tb-bearing balancers (FM7-TbA and CyO-TbA) for more than 30 generations and found that the Tb1 transgene they carry is stable. Thus, these new Tb-tagged balancers are particularly useful for balancing lethal mutations and distinguish homozygous mutant larvae from their heterozygous siblings.
Tubby-tagged balancers for the Drosophila X and second chromosomes / Lattao, Ramona; Bonaccorsi, Silvia; Xiao, Guan; S. a., Wasserman; Gatti, Maurizio. - In: FLY. - ISSN 1933-6934. - STAMPA. - 5:4(2011), pp. 369-370. [10.4161/fly.5.4.17283]
Tubby-tagged balancers for the Drosophila X and second chromosomes
LATTAO, RAMONA;BONACCORSI, Silvia;GATTI, MAURIZIO
2011
Abstract
We generated FM7a and CyO balancer chromosomes bearing a Tubby(1) (Tb(1)) dominant transgene. Flies heterozygous for these FM7a and CyO derivatives exhibit a phenotype undistinguishable from that elicited by the Tb1 mutation associated with the TM6B balancer. We tested two of these Tb-bearing balancers (FM7-TbA and CyO-TbA) for more than 30 generations and found that the Tb1 transgene they carry is stable. Thus, these new Tb-tagged balancers are particularly useful for balancing lethal mutations and distinguish homozygous mutant larvae from their heterozygous siblings.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.