Human erythrocytes extensively crosslinked with glutaraldehyde display very high mechanical and chemical resistance, but still bind oxygen reversibly. Depending on the initial reaction conditions the oxygen affinity of fixed erythrocytes is either high or (partially) low, the binding curve being always non cooperative. Cells which are extensively crosslinked but still active may find a number of biotechnological applications; fixed erythrocytes may (for example) be investigated as possibe blood substitutes.
Human erythrocytes cross-linked with glutaraldehyde general properties and significance as a blood substitute / Bellelli, Andrea; Benedetti, Pl; Coletta, M; Ippoliti, R; Brunori, Maurizio. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 156:(1988), pp. 970-977. [10.1016/S0006-291X(88)80939-2]
Human erythrocytes cross-linked with glutaraldehyde general properties and significance as a blood substitute.
BELLELLI, Andrea;BRUNORI, Maurizio
1988
Abstract
Human erythrocytes extensively crosslinked with glutaraldehyde display very high mechanical and chemical resistance, but still bind oxygen reversibly. Depending on the initial reaction conditions the oxygen affinity of fixed erythrocytes is either high or (partially) low, the binding curve being always non cooperative. Cells which are extensively crosslinked but still active may find a number of biotechnological applications; fixed erythrocytes may (for example) be investigated as possibe blood substitutes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
