Nowadays, testosterone remains a highly abused anabolic androgenic steroid for the practice of doping. There are many causes that make its detection particularly complicated,since it is an endogenous compound and its concentration in spot urine samples is highly variable (inter and intra-individuals) Many approaches have been proposed for the detection of T misuse. From the classical T/E (reference population, WADA) to personalize the references, the individual became its own reference, using an univariate or multivariate analysis approach for the statistical discrimination of steroid profile in the detection of abnormal profiles. None of this excludes previous approaches, all of them use the anabolic steroid profile, and are part of the WADA effort to establish and improved the athlete biological passport. To improve the urinary steroid profile one way is to extend its evaluation with the inclusion of new biomarkers.
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