Single channel recording patch-clamp technique was used in the mouse to compare the acetylcholine (ACh)-induced channel behaviour between normal and dystrophic myotubes. While open time and slope conductance were equivalent, ACh-induced channel opening frequency was more than 4-fold reduced in dystrophic compared to normal myotubes. In addition, the steady-state phosphorylation of the ACh receptor (AChR), tested by immunoprecipitation of 32P-labeled cells, indicated that the α-subunit was more heavily phosphorylated in the dystrophic myotubes. We propose that the degree of α-subunit phosphorylation of the AChR, which parallels the reduced AChR-channel opening probability, determines desensitization of the AChR in dystrophic myotubes.
REDUCED ACETYLCHOLINE-INDUCED CHANNEL ACTIVITY IN DYSTROPHIC MOUSE MYOTUBES / Grassi, Francesca; Giulio, Cossu; Eusebi, Fabrizio. - In: JOURNAL OF THE NEUROLOGICAL SCIENCES. - ISSN 0022-510X. - STAMPA. - 84:1(1988), pp. 77-86. [10.1016/0022-510x(88)90176-1]
REDUCED ACETYLCHOLINE-INDUCED CHANNEL ACTIVITY IN DYSTROPHIC MOUSE MYOTUBES
GRASSI, Francesca;EUSEBI, Fabrizio
1988
Abstract
Single channel recording patch-clamp technique was used in the mouse to compare the acetylcholine (ACh)-induced channel behaviour between normal and dystrophic myotubes. While open time and slope conductance were equivalent, ACh-induced channel opening frequency was more than 4-fold reduced in dystrophic compared to normal myotubes. In addition, the steady-state phosphorylation of the ACh receptor (AChR), tested by immunoprecipitation of 32P-labeled cells, indicated that the α-subunit was more heavily phosphorylated in the dystrophic myotubes. We propose that the degree of α-subunit phosphorylation of the AChR, which parallels the reduced AChR-channel opening probability, determines desensitization of the AChR in dystrophic myotubes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
