We generated monoclonal antibodies (MoAbs) BF11 and VNH9 against Candida rugosa lipase (CRL), one of the most widely used lipolytic enzymes. The antibodies, both of the IgG(1) isotype recognize specie specific and distinct antigenic determinants shared by different CRL isoforms present both in the native and in the denaturated form of the enzyme. The CRL enzyme maintained its enzymatic activity even when immunocomplexed with MoAb BF11 and VNH9. These novel reagents are of interest for their possible application as immobilization and purification systems, and during fermentation processes. (C) 2004 Elsevier B.V. All rights reserved.
Monoclonal antibodies against Candida rugosa lipase / RAHIMI KOSHKAKI, Hassan; Soro, Simonetta; Rughetti, Aurelia; Palocci, Cleofe; Mauro, Biffoni; Serena, Barachini; Federica, Taurino; Cernia, Enrico; Frati, Luigi; Nuti, Marianna. - In: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC. - ISSN 1381-1177. - STAMPA. - 28:2-3(2004), pp. 71-74. [10.1016/j.molcatb.2004.01.002]
Monoclonal antibodies against Candida rugosa lipase
RAHIMI KOSHKAKI, HASSAN;SORO, Simonetta;RUGHETTI, Aurelia;PALOCCI, Cleofe;CERNIA, Enrico;FRATI, Luigi;NUTI, Marianna
2004
Abstract
We generated monoclonal antibodies (MoAbs) BF11 and VNH9 against Candida rugosa lipase (CRL), one of the most widely used lipolytic enzymes. The antibodies, both of the IgG(1) isotype recognize specie specific and distinct antigenic determinants shared by different CRL isoforms present both in the native and in the denaturated form of the enzyme. The CRL enzyme maintained its enzymatic activity even when immunocomplexed with MoAb BF11 and VNH9. These novel reagents are of interest for their possible application as immobilization and purification systems, and during fermentation processes. (C) 2004 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
