Junctional transfer of ions in monolayer primary cultures of Sertoli cells has been studied by using conventional techniques. Cells were extensively electrically coupled. Membrane voltage displacements caused by the injected current pulses were measured at variable interelectrode distance. Data analyses were based on a thin-sheet model for current flow )Jongsma and van Tijn, 1972) and yielded a generalized space constant of 248 um and an internal resistivity of 1.7 k0hm.cm in standard medium. Electrical coupling was inhibited by A23187 Ca2+-ionophore (5 uM). © 1985 Springer-Verlag.
Cell-to-cell communication in cultured Sertoli cells / Fabrizio, Eusebi; Grassi, Francesca; Fratamico, Guiseppe; Susanna, Dolci; Marco, Conti; Mario, Stefanini. - In: PFLÜGERS ARCHIV. - ISSN 0031-6768. - STAMPA. - 404:4(1985), pp. 382-384. [10.1007/bf00585353]
Cell-to-cell communication in cultured Sertoli cells
GRASSI, Francesca;
1985
Abstract
Junctional transfer of ions in monolayer primary cultures of Sertoli cells has been studied by using conventional techniques. Cells were extensively electrically coupled. Membrane voltage displacements caused by the injected current pulses were measured at variable interelectrode distance. Data analyses were based on a thin-sheet model for current flow )Jongsma and van Tijn, 1972) and yielded a generalized space constant of 248 um and an internal resistivity of 1.7 k0hm.cm in standard medium. Electrical coupling was inhibited by A23187 Ca2+-ionophore (5 uM). © 1985 Springer-Verlag.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
