Ochratoxin A (OTA), a mycotoxin with nephrotoxic, nephrocarcinogenic, teratogenic and immunosuppressive properties, has been detected in different kinds of foods and beverages, including grape juice and wine. Among the OTA-producing fungal species, Aspergillus carbonarius shows the highest ochratoxigenic potential in grapes. The objective of this research was to investigate the presence of A. carbonarius in grapes and of OTA in wine. To detect the presence of A. carbonarius in grapes, two PCR assays were developed using species-specific primers designed on sequences of: (i) Internal Transcribe Spacers of rDNA units (ITS), used to carry out phylogenetic studies; (ii) polyketide synthases (PKSs) genes responsible for the biosynthesis of ochracotoxin A. Currently, most of the methods available for the determination of OTA in wine are based on an extraction step, a cleanup passage and assessment by High Performance Liquid Chromatografy Chromatografy (HPLC) with fluorescence detection. In this work we investigated the performance of a system based on hydrogenated amorphous silicon photosensors for the early detection of Ochratoxin A, based on the measurement of the photocurrent induced in a hydrogenated amorphous silicon (a-Si:H) photodiode by the fluorescence of the mycotoxin excited by UV radiation. The mycotoxin is deposited on a thin layer chromatographic plate and aligned with the sensor. The photocurrent value is proportional to the mycotoxin quantity deposited.
EARLY DETECTION OF OCHRATOXIGENIC FUNGI ON GRAPE AND OCHRATOXIN A IN WINE / P., De Rossi; Reverberi, Massimo; Alessandra, Ricelli; Caputo, Domenico; DE CESARE, Giampiero; Nascetti, Augusto; Scipinotti, Riccardo; Fanelli, Corrado. - In: JOURNAL OF PLANT PATHOLOGY. - ISSN 1125-4653. - STAMPA. - 89:3 (supplement)(2007), pp. S16-S16. (Intervento presentato al convegno XIV NATIONAL MEETING tenutosi a Perugia, Italia nel 19-21 settembre 2007).
EARLY DETECTION OF OCHRATOXIGENIC FUNGI ON GRAPE AND OCHRATOXIN A IN WINE
REVERBERI, Massimo;CAPUTO, Domenico;DE CESARE, Giampiero;NASCETTI, Augusto;SCIPINOTTI, RICCARDO;FANELLI, Corrado
2007
Abstract
Ochratoxin A (OTA), a mycotoxin with nephrotoxic, nephrocarcinogenic, teratogenic and immunosuppressive properties, has been detected in different kinds of foods and beverages, including grape juice and wine. Among the OTA-producing fungal species, Aspergillus carbonarius shows the highest ochratoxigenic potential in grapes. The objective of this research was to investigate the presence of A. carbonarius in grapes and of OTA in wine. To detect the presence of A. carbonarius in grapes, two PCR assays were developed using species-specific primers designed on sequences of: (i) Internal Transcribe Spacers of rDNA units (ITS), used to carry out phylogenetic studies; (ii) polyketide synthases (PKSs) genes responsible for the biosynthesis of ochracotoxin A. Currently, most of the methods available for the determination of OTA in wine are based on an extraction step, a cleanup passage and assessment by High Performance Liquid Chromatografy Chromatografy (HPLC) with fluorescence detection. In this work we investigated the performance of a system based on hydrogenated amorphous silicon photosensors for the early detection of Ochratoxin A, based on the measurement of the photocurrent induced in a hydrogenated amorphous silicon (a-Si:H) photodiode by the fluorescence of the mycotoxin excited by UV radiation. The mycotoxin is deposited on a thin layer chromatographic plate and aligned with the sensor. The photocurrent value is proportional to the mycotoxin quantity deposited.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
