Several procedures for immobilization of pyruvateoxidase by chemical bonding are reported. Attachment to nylon net was unsatisfactory in terms of yield and stability. Polyazetidine, a new commercially available prepolymer and a new nylon membrane with surface carboxyl groups provided good long-term stability, up to 30 days in the case of the nylon membrane. Under optimal conditions of solution preparation (0.1 mmol 1-1 thiamine pyrophosphate, 0.5 mmol 1-1 phosphate, 2.5 mmol 1-1 calcium and 0.04 mol 1-1 Tris buffer pH 7.0), linear calibration graphs were obtained until the lowest concentration of 10-3 mmol 1-1pyruvate and amperometricsensors based on these membranes. With careful standardization, the procedures were suitable for application to blood serum.
AMPEROMETRIC SENSOR FOR PYRUVATE WITH IMMOBILIZED PYRUVATE OXIDASE / M., Mascini; Mazzei, Franco. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 192:1(1987), pp. 9-16. [10.1016/s0003-2670(00)85683-4]
AMPEROMETRIC SENSOR FOR PYRUVATE WITH IMMOBILIZED PYRUVATE OXIDASE
MAZZEI, Franco
1987
Abstract
Several procedures for immobilization of pyruvateoxidase by chemical bonding are reported. Attachment to nylon net was unsatisfactory in terms of yield and stability. Polyazetidine, a new commercially available prepolymer and a new nylon membrane with surface carboxyl groups provided good long-term stability, up to 30 days in the case of the nylon membrane. Under optimal conditions of solution preparation (0.1 mmol 1-1 thiamine pyrophosphate, 0.5 mmol 1-1 phosphate, 2.5 mmol 1-1 calcium and 0.04 mol 1-1 Tris buffer pH 7.0), linear calibration graphs were obtained until the lowest concentration of 10-3 mmol 1-1pyruvate and amperometricsensors based on these membranes. With careful standardization, the procedures were suitable for application to blood serum.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
