Acetylcholine treatment of [3H]inositol pre-labelled cultured chick embryo myotubes results in the stimulation of phosphatidylinositol breakdown, as shown by the measurement of inositol-1-phosphate accumulating in the presence of lithium. The described effect is dependent on agonist concentration and incubation time, and is inhibited by tubocurarine and α-bungarotoxin. The activation of phosphatidylinositol breakdown by acetylcholine at extrajunctional nicotinic receptors is likely to be involved in the modulation of the functional activity of the receptor. © 1985.
Acetylcholine stimulates phosphatidylinositol turnover at nicotinic receptors of cultured myotubes / Adamo, Sergio; Bianca Maria, Zani; Nervi, Clara; Maria Immacolata, Senni; M., Molinaro; Fabrizio, Eusebi. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 190:1(1985), pp. 161-164. [10.1016/0014-5793(85)80449-x]
Acetylcholine stimulates phosphatidylinositol turnover at nicotinic receptors of cultured myotubes
ADAMO, Sergio;NERVI, Clara;
1985
Abstract
Acetylcholine treatment of [3H]inositol pre-labelled cultured chick embryo myotubes results in the stimulation of phosphatidylinositol breakdown, as shown by the measurement of inositol-1-phosphate accumulating in the presence of lithium. The described effect is dependent on agonist concentration and incubation time, and is inhibited by tubocurarine and α-bungarotoxin. The activation of phosphatidylinositol breakdown by acetylcholine at extrajunctional nicotinic receptors is likely to be involved in the modulation of the functional activity of the receptor. © 1985.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
