Using PCR we found that G protein-coupled receptor kinase4 (GRK4) mRNA is expressed only in brain out of several tissues tested. In the brain two amplification products were generated. Sequence analysis revealed that the two fragments differed only by the presence or absence of an in-frame-sequence of 96 bp/32 aminoacids, located near the N-terminal of the kinase. This demonstrates the existence of two isoforms of GRK4 which were named GRK4A and GRK4B in the presence or absence of the insert, respectively. This is the first evidence that, within the GRKs gene family, different isoforms do exist.
Two isoforms of G protein-coupled receptor kinase 4 identified by molecular cloning / M., Sallese; M. S., Lombardi; DE BLASI, Antonio. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 199:2(1994), pp. 848-854. [10.1006/bbrc.1994.1306]
Two isoforms of G protein-coupled receptor kinase 4 identified by molecular cloning
DE BLASI, ANTONIO
1994
Abstract
Using PCR we found that G protein-coupled receptor kinase4 (GRK4) mRNA is expressed only in brain out of several tissues tested. In the brain two amplification products were generated. Sequence analysis revealed that the two fragments differed only by the presence or absence of an in-frame-sequence of 96 bp/32 aminoacids, located near the N-terminal of the kinase. This demonstrates the existence of two isoforms of GRK4 which were named GRK4A and GRK4B in the presence or absence of the insert, respectively. This is the first evidence that, within the GRKs gene family, different isoforms do exist.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
