We report that upon muscarinic stimulation of SK-N-BE(2) human neuroblastoma cells, the extent of phosphoinositide-derived diacylglycerol (DG) conversion to phosphatidic acid (PA), operated by a DG kinase, is dependent on the potency of receptor stimulation and correlates with the reduction of phosphatidylinositol 4,5-bisphosphate mass. Evidence is provided that agonist-evoked Ca2+ mobilization or protein kinase activation are not key events in triggering receptor-generated DG conversion to PA; furthermore, the phenomenon is compartmentalized, namely it occurs within a topologically restricted area that is poorly accessible to DG artificially generated by cell treatment with bacterial phosphatidylinositol-specific phospholipase C. Possible mechanisms driving regulation of the DG kinase operating in the transduction system investigated are discussed.

Phosphoinositide-derived diacylglycerol conversion to phosphatidic acid is a receptor-dependent and compartmentalized phenomenon in human neuroblastoma / Limatola, Cristina; Laura, Pacini; Irene, Ricci; Angelo, Spinedi. - In: NEUROSCIENCE LETTERS. - ISSN 0304-3940. - STAMPA. - 219:2(1996), pp. 127-130. [10.1016/s0304-3940(96)13200-6]

Phosphoinositide-derived diacylglycerol conversion to phosphatidic acid is a receptor-dependent and compartmentalized phenomenon in human neuroblastoma

LIMATOLA, Cristina;
1996

Abstract

We report that upon muscarinic stimulation of SK-N-BE(2) human neuroblastoma cells, the extent of phosphoinositide-derived diacylglycerol (DG) conversion to phosphatidic acid (PA), operated by a DG kinase, is dependent on the potency of receptor stimulation and correlates with the reduction of phosphatidylinositol 4,5-bisphosphate mass. Evidence is provided that agonist-evoked Ca2+ mobilization or protein kinase activation are not key events in triggering receptor-generated DG conversion to PA; furthermore, the phenomenon is compartmentalized, namely it occurs within a topologically restricted area that is poorly accessible to DG artificially generated by cell treatment with bacterial phosphatidylinositol-specific phospholipase C. Possible mechanisms driving regulation of the DG kinase operating in the transduction system investigated are discussed.
1996
carbachol; cultured; diacylglycerol; diacylglycerol kinase; diglycerides; humans; metabolism; metabolism/pathology; muscarinic; muscarinic agonists; neuroblastoma; pharmacology; phosphatidic acid; phosphatidic acids; phosphatidylinositols; physiology; receptors; tissue distribution; tumor cells
01 Pubblicazione su rivista::01a Articolo in rivista
Phosphoinositide-derived diacylglycerol conversion to phosphatidic acid is a receptor-dependent and compartmentalized phenomenon in human neuroblastoma / Limatola, Cristina; Laura, Pacini; Irene, Ricci; Angelo, Spinedi. - In: NEUROSCIENCE LETTERS. - ISSN 0304-3940. - STAMPA. - 219:2(1996), pp. 127-130. [10.1016/s0304-3940(96)13200-6]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/452974
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