We characterized the role of adenosine receptor (AR) subtypes in the modulation of glutamatergic neurotransmission by the chemokine fractalkine (CX3CL1) in mouse hippocampal CA1 neurons. CX3CL1 causes a reversible depression of excitatory postsynaptic current (EPSC), which is abolished by the A3R antagonist MRS1523, but not by A1R (DPCPX) or A2AR (SCH58261) antagonists. Consistently, CX3CL1-induced EPSC depression is absent in slices from A3R-/- but not A1R-/- or A2AR-/- mice. Further, A3R stimulation causes similar EPSC depression. In cultured neurons, CX3CL1-induced depression of AMPA current shows A1R-A3R pharmacology. We conclude that glutamatergic depression induced by released adenosine requires the stimulation of different ARs. © 2010 Elsevier B.V.
CX3CL1-induced modulation at CA1 synapses reveals multiple mechanisms of EPSC modulation involving adenosine receptor subtypes / S., Piccinin; DI ANGELANTONIO, Silvia; A., Piccioni; R., Volpini; G., Cristalli; B. b., Fredholm; Limatola, Cristina; Eusebi, Fabrizio; Ragozzino, Davide Antonio. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 224:1-2(2010), pp. 85-92. [10.1016/j.jneuroim.2010.05.012]
CX3CL1-induced modulation at CA1 synapses reveals multiple mechanisms of EPSC modulation involving adenosine receptor subtypes
DI ANGELANTONIO, SILVIA;LIMATOLA, Cristina;EUSEBI, Fabrizio;RAGOZZINO, Davide Antonio
2010
Abstract
We characterized the role of adenosine receptor (AR) subtypes in the modulation of glutamatergic neurotransmission by the chemokine fractalkine (CX3CL1) in mouse hippocampal CA1 neurons. CX3CL1 causes a reversible depression of excitatory postsynaptic current (EPSC), which is abolished by the A3R antagonist MRS1523, but not by A1R (DPCPX) or A2AR (SCH58261) antagonists. Consistently, CX3CL1-induced EPSC depression is absent in slices from A3R-/- but not A1R-/- or A2AR-/- mice. Further, A3R stimulation causes similar EPSC depression. In cultured neurons, CX3CL1-induced depression of AMPA current shows A1R-A3R pharmacology. We conclude that glutamatergic depression induced by released adenosine requires the stimulation of different ARs. © 2010 Elsevier B.V.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.