Cellular inclusions containing ubiquitin and alpha-synuclein were observed in PC12 cells treated with metamphetamine (MA). To study the possible involvement of beta-arrestin in inclusion formation, we treated PC12 cells with MA for different times and analyzed the ubiquitin proteosome pathway (UPP). We found that beta-arrestin is ubiquitinated in the MA-treated PC12 cell line. The involvement of beta-arrestin in UPP was further supported by electron microscopy and by confocal microscopy, which documented the presence of beta-arrestin in these Lewy body-like inclusions. Our experiments reveal an interesting and previously unappreciated connection between beta-arrestin and ubiquitination and suggest that beta-arrestin could be involved in the development of the inclusion bodies.
Presence of beta-arrestin in cellular inclusions in metamphetamine-treated PC12 cells / DE BLASI, Antonio; L., Capobianco; Iacovelli, Luisa; P., Lenzi; M., Ferrucci; G., Lazzeri; F., Fornai; A., Picascia. - In: NEUROLOGICAL SCIENCES. - ISSN 1590-1874. - STAMPA. - 24:3(2003), pp. 164-165. [10.1007/s10072-003-0111-5]
Presence of beta-arrestin in cellular inclusions in metamphetamine-treated PC12 cells.
DE BLASI, ANTONIO;IACOVELLI, LUISA;
2003
Abstract
Cellular inclusions containing ubiquitin and alpha-synuclein were observed in PC12 cells treated with metamphetamine (MA). To study the possible involvement of beta-arrestin in inclusion formation, we treated PC12 cells with MA for different times and analyzed the ubiquitin proteosome pathway (UPP). We found that beta-arrestin is ubiquitinated in the MA-treated PC12 cell line. The involvement of beta-arrestin in UPP was further supported by electron microscopy and by confocal microscopy, which documented the presence of beta-arrestin in these Lewy body-like inclusions. Our experiments reveal an interesting and previously unappreciated connection between beta-arrestin and ubiquitination and suggest that beta-arrestin could be involved in the development of the inclusion bodies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
