Manyenzymatic reactions yield volatile products either directly or by cascade sequences, so it seems possible thathead-space chromatographymight be used to determine enzymatic activity. The activityof urinary kallikrein, as arginineesterase, has been determinedin this way by using the N(alpha)-acetyl-L-phenylalanyl-L-arginineethyl ester as substrate and measuring the ethanol yielded onincubation for 10 min at 30 degrees, followed by quenchingof the reaction. The method has been applied to aqueous solutions and urine
Head-space chromatography in determinationof enzymatic activity: microdeterminationof the urinary kallikrein as arginine esterases / Curini, Roberta; DE ANGELIS CURTIS, Simonetta Carla Benedett; G., D’Ascenzo; Lagana', Aldo. - In: TALANTA. - ISSN 0039-9140. - STAMPA. - 36:11(1989), pp. 1087-1090. [10.1016/0039-9140(89)80005-0]
Head-space chromatography in determinationof enzymatic activity: microdeterminationof the urinary kallikrein as arginine esterases
CURINI, Roberta;DE ANGELIS CURTIS, Simonetta Carla Benedett;LAGANA', Aldo
1989
Abstract
Manyenzymatic reactions yield volatile products either directly or by cascade sequences, so it seems possible thathead-space chromatographymight be used to determine enzymatic activity. The activityof urinary kallikrein, as arginineesterase, has been determinedin this way by using the N(alpha)-acetyl-L-phenylalanyl-L-arginineethyl ester as substrate and measuring the ethanol yielded onincubation for 10 min at 30 degrees, followed by quenchingof the reaction. The method has been applied to aqueous solutions and urineI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
